Selected Publications

    2011

  1. Liu, X.Q. and Wang, H.W. (2011) Single particle electron microscopy reconstruction of the exosome complex using the random conical tilt method. J. Vis. Exp., doi: 10.3791/2574.
  2. Ramey, V.H., Wang, H.W., Nakajima, Y., Wong, A., Liu, J., Drubin, D., Barnes, G., and Nogales, E. (2011) The Dam1 ring binds to the E-hook of tubulin and diffuses along the microtubule. Mol. Biol. Cell, 22(4):457-466
  3. Wu, X., Shen, Q.T., Oristian, D.S., Lu, C.P., Zheng, Q., Wang, H.W., and Fuchs, E. (2011) Skin stem cells orchestrate directional migration by regulating microtubule-ACF7 connections through GSK3b. Cell, 144(3):341-352

    4. 2010

  4. Yang, H., Wang, J., Jia, X., McNatt, M.W., Zang, T., Pan, B., Meng, W., Wang, H.W., Bieniasz, P.D., and Xiong, Y. (2010) Structural insight into the mechanisms of enveloped virus tethering by tetherin. Proc. Natl. Acad. Sci. USA, 107(43):18428-18432
  5. Nogales, E., Ramey, V.H., and Wang, H.W. (2010) Cryo-EM studies of microtubule structural intermediates and kinetochore-microtubule interactions. Methods Cell Biol., 95:129-156

    6. 2009

  6. Wu, Z., Wang, H.W., Mu, W., Quyang Z., Nogales, E., and Xing, J. (2009) Simulations of tubulin sheet polymers as possible structural intermediates in microtubule assembly. PLoS ONE, 4(10):e7291
  7. Wang, H.W.*, Noland, C., Siridechadilok, B., Taylor, D.W., Ma, E., Felderer, K., Doudna, J.A.* and Nogales, E.*(2009) Structural insights into RNA processing by the human RISC-loading complex. Nat. Struct. Mol. Biol., 16(11):1148-1153(* corresponding authors)
  8. Ramey, V.H., Wang, H.W. and Nogales. E. (2009) Ab initio reconstruction of helical samples with heterogeneity, disorder and coexisting symmetries. J. Struct. Biol., 167(2):97-105
  9. Shen, Q.T., Bai, X.C., Chang, L.F., Wu, Y., Wang, H.W. and Sui, S.F. (2009) The bowl-shaped oligomeric structure on membranes as DegP's new functional form in protein quality control. Proc. Natl. Acad. Sci. USA, 106:4858-4863

    10. 2008

  10. Wang, H.W., Long, S., Ciferri, C., Westermann, S., Drubin, D., Barnes, G. and Nogales, E. (2008) Architecture and flexibility of the yeast Ndc80 kinetochore complex. J. Mol. Biol., 383(4):894-903

    11. 2007

  11. Wang, H.W.*, Wang, J., Ding, F., Callahan, K., Bratkowski, M.A., Bulter, J.S., Nogales, E. and Ke, A.* (2007)Architecture of the yeast Rrp44-exosome complex suggests routes of RNA recruitment for 3’-end processing. Proc. Natl. Acad. Sci. USA, 104:16844-16849 (* corresponding authors)
  12. Wang, H.W., Ramey, V.H., Westermann, S., Leschziner, A., Welburn, J.P.I., Nakajima, Y., Drubin, D.G., Barnes, G. and Nogales, E. (2007) Architecture of the Dam1 kinetochore ring complex and implications for microtubule-driven assembly and force-coupling mechanisms. Nat. Struct. Mol. Biol., 14:721-726

    13. 2006

  13. Nogales, E. and Wang, H.W. (2006) Structural mechanisms underlying nucleotide-dependent self-assembly of tubulin and its relatives. Curr. Opin. Struct. Biol., 16:221-229
  14. Nogales, E. and Wang, H.W. (2006) Structural intermediates in microtubule assembly and disassembly: how and why? Curr. Opin. Cell Biol., 18:179-284
  15. Westermann, W., Wang, H.W., Avila-Sakar, A., Drubin, D.G., Nogales, E. and Barnes, G. (2006) The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature, 440:565-563

    16. 2005

  16. Wang, H.W.*, Long, S., Finley, K.R. and Nogales, E. (2005) Assembly of GMPCPP-bound tubulin into helical ribbons and tubes and effect of colchicine. Cell Cycle., 4(9):1157-1160 (* corresponding author)
  17. Wang, H.W. and Nogales,E. (2005) The nucleotide-dependent bending flexibility of tubulin regulates microtubule assembly. Nature, 435:911-915
  18. Wang, H.W. and Nogales, E. (2005) An iterative Fourier-Bessel algorithm for reconstruction of helical structures with severe Bessel overlap. J. Struct. Biol., 149:65-78
  19. Westermann, S., Avilia-Saker, A., Wang, H.W., Niederstrasser, H., Wong, J., Drubin, D.G., Nogales, E. and Barnes, G. (2005). Formation of a dynamic kinetochore-microtubule interface through assembly of the Dam1 ring complex. Mol. Cell., 17(2):277-290

    20. 2004 and earlier

  20. Nogales, E., Wang, H.W. and Niederstrasser, H. (2003) Tubulin rings: which way do they curve? Curr. Opin. Struct. Biol., 13:256-261
  21. Chen, Y., Lu, Y.J., Wang, H.W., Yuan, S., Chang, Z., and Sui, S.F. (2003) Two-dimensional crystallization of a small heat shock protein HSP16.3 on lipid layer. Biochem. Biophys. Res. Commun., 310(2):360-366
  22. Wu, Y., Wang, H.W., Ji, S.R. and Sui, S.F. (2003)Two-dimensional crystallization of rabbit C-reactive protein monomeric subunits. Acta. Crystallogr. D. Biol. Crystallogr., 59(Pt 5):922-926
  23. Wang, H.W., Chen, Y., Yang, H., Chen, X., Duan, M.X., Tai, P.C. and Sui, S.F. (2003) Ring-like pore structures of SecA: implication for bacteria protein-conducting channels. Proc. Natl. Acad. Sci. USA, 100:4221-4226
  24. Wu, Y., Ji, S.R., Wang, H.W. and Sui, S.F. (2002)Study of the spontaneous dissociation of rabbit C-reactive protein. Biochemistry (Mosc), 67(12):1377-1382
  25. Wang, H.W., Wu, Y., Chen, Y. and Sui, S.F. (2002)Polymorphism of structural forms of C-reactive protein. Int. J. Mol. Med., 9(6):665-671
  26. Wang, H.W. and Sui, S.F. (2001) Dissociation and Subunit Rearrangement of Membrane-Bound Human C-Reactive Proteins. Biochem. Biophys. Res. Commun., 288(1):75-79
  27. Wang, H.W. and Sui, S.F. (2001)Two-Dimensional Assembly of Pentameric Rabbit C-Reactive Proteins on Lipid Monolayers. J. Struct. Biol., 134(1):46-55
  28. Wang, H.W., Lu, Y.J., Li, Y.J., Liu, S. Wang, D.N. and Sui, S.F. (2000)Trimeric ring-like structure of ArsA ATPase. FEBS Lett., 469(1):105-110
  29. Zhou, H., Wang, H.W., Zhu, K., Sui, S.F., Xu, P., Yang, S.F. and Li, N. (1999)The multiple roles of conserved arginine 286 of 1-aminocyclopropane-1-carboxylate synthase. Coenzyme binding, substrate binding, and beyond. Plant Physiol., 121(3):913-919
  30. Wang, H.W and Sui, S.F. (1999)Pentameric two-dimensional crystallization of rabbit C-reactive protein on lipid monolayers. J. Struct. Biol., 127(3):283-286
  31. Mi, L.Z., Wang, H.W. and Sui, S.F. (1997)Interaction of rabbit C-reactive protein with phospholipid monolayers studied by microfluorescence film balance with an externally applied electric field. Biophys J., 73(1):446-451

    32. full list of publications