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Baocheng Pan, Ph.D. Center for Structural Biology Phone:
(203) 432-5625 Email: pan@csb.yale.edu |
Interaction Mechanism of T7 RNAP with DNA and RNA
Summary:
Bacteriophage T7 RNA polymerase (T7 RNAP) is a single-subunit enzyme (molecular weight of 99 kD) that is capable of transcribing the complete T7 phage DNA. It is homologous to eukaryotic mitochondrial, chloroplast, other phagelike RNAPs and also to the well-studied family I of DNA polymerases. T7 RNAP has advantages for crystallographic studies in its smaller size than the multi-subunit prokaryotic and eukaryotic polymerases and its well-characterized interactions with promoter DNA. The crystal structures of T7 RNAP (1), one of its initiation complex (2) and its elongation complex (3) have already been determined. These structures provide rich information about the conformation of the polymerase and its interaction mechanism with DNA promoters and RNA transcripts. Further crystallographic studies of various constructs with T7 RNAP will be useful in illustrating the whole picture of the interaction mechanism of the polymerase.
References:
1. Sousa, R., Chung, Y. J., Rose, J. P. & Wang, B.-C. (1993) Nature 364, 593-599.
2. Cheethan, G. M. & Steitz, T. A. (1999) Science 286, 2305-2309.
3. Yin,
Y. W. & Steitz, T. A. (2002) Science 298, 1387-1395.
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Recent Publication:
Pan, B., Xiong, Y., Shi, K. & Sundaralingam, M. (2003) Crystal structure of a bulged RNA tetraplex at 1.1Å resolution: implications for a novel binding site for RNA tetraplex. Structure 11, 1423-1430.
Pan, B., Xiong, Y., Shi, K. & Sundaralingam, M. (2003) An eight-stranded helical fragment in RNA crystal structure: implicastions for tetraplex interaction. Structure 11, 825-831.
Pan, B., Xiong, Y., Shi, K. Deng, J. & Sundaralingam, M. (2003) Crystal structure of an RNA purine-rich tetraplex containing adenine-tetrads: implications for specific binding in RNA tetraplexes. Structure 11, 815-823.
Ravelli, R. B. G. Leiros, H.-K. S., Pan, B., Caffrey, M. & McSweeney, S. (2003) Specific radiation damage can be used to solve macromolecular crystal structures. Structure 11, 217-224.
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