Bob Grodzicki Research Associate Center for Structural Biology Department of Molecular Biophysics & Biochemistry Yale University 266 Whitney Avenue / Bass 415 P.O. Box 208114 New Haven, CT 06520-8114 Phone:  (203) 432-5627 FAX:  (203) 432-3282 Email: rlg {at} csb.yale.edu

Summary:

I am trying to determine how various ribosomal structures contribute to the peptidyl transferase reaction.
 

Selected Publications:

Cascio M, Shenkel S, Grodzicki RL, Sigworth FJ, Fox RO: Functional Reconstitution and Characterization of Recombinant Human alpha-1-Glycine Receptors. J Biol Chem, 276(4), 20981-20988, 2001.

Cascio M, Schoppa NE, Grodzicki RL, Sigworth FJ, Fox RO: Functional Expression and Purification of a Homomeric Human alpha-1 Glycine Receptor in Baculovirus-infected Insect Cells. J Biol Chem, 268(29), 22135-22142, 1993.

Grodzicki RL, Steere AC: Comparison of immunoblotting and indirect enzyme-linked immunosorbent assay using different antigen preparations for diagnosing early Lyme disease. J Inf Dis, 157(4), 790-797, 1988.

Shrestha M, Grodzicki RL, Steere AC: Diagnosing early Lyme disease. Am J Med, 78, 235-240, 1985.

Ackermann R, Kabatski J, Boisten HP, Steere AC, Grodzicki RL, Hartung S, Runne U: Ixodes ricinus spirochete and European erythema chronicum migrans disease. Yale J Biol Med, 57, 573-580, 1984.

Craft JE, Grodzicki RL, Shrestha M, Fischer DK, Garcia-Blanco M, Steere AC: The antibody response in Lyme disease. Yale J Biol Med, 57, 589-594, 1984.

Steere AC, Grodzicki RL, Craft JE, Shrestha M, Kornblatt AN, Malawista SE: Recovery of Lyme disease spirochetes from patients. Yale J Biol Med, 57, 557-560, 1984.

Craft JE, Grodzicki RL, Steere AC: The antibody response in Lyme disease: Evaluation of diagnostic tests. J Inf Dis, 149, 789-795, 1984.

Steere AC, Grodzicki RL, Kornblatt AN, Craft JE, Barbour AG, Burgdorfer W, Schmid GP, Johnson E, and Malawista SE: The spirochetal etiology of Lyme disease. N Engl J Med, 308, 733-740, 1983.

Rahn DW, Steere AC, Grodzicki RL, Levine JH, and Hardin JA: Evidence for Pseudomonas antigen in immune complexes in Pseudomonas osteomylelitis. Arthritis Rheum, 25, 1403-1408, 1982.

Whipple CA, Grodzicki RL, Hourihan J, Salhanick HA. Effects of cholesterol side chain cleavage inhibitors on 11 beta-hydroxylation: linkage of mitochondrial oxygenase systems. Steroids, 37(6), 673-679, 1981.

Rao VS, Bennett JA, Grodzicki RL, Mitchell MS. Suppressor T cells induced by soluble immune complexes can adoptively transfer inhibition of cytophilic antibody receptors on macrophages. Cell Immunol, 46(2), 227-238, 1979.

Rao VS, Grodzicki RL, Mitchell MS. Specific in vivo inhibition of macrophage receptors for cytophilic antibody by soluble immune complexes. Cancer Res, 39(1), 174-182, 1979.

 

Some papers relevant to my work:

Moore PB and Steitz TA. After the ribosome structures: How does peptidyl transferase work? RNA, 9, 155-159, 2003.

Hansen JL, Schmeing TM, Moore PB, and Steitz TA. Structural insights into peptide bond formation. PNAS, 99(18), 11670-11675, 2002.

Schmeing TM, et al. A pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits. Natur Structural Biol, 9(3), 225-230, 2002.

Semra K and Green R. Osmolytes stimulate the reconstitution of functional 50S ribosomes from in vitro transcripts of Escherichia coli 23S rRNA. RNA, 8, 401-411, 2002.

Ramakrishnan V. Ribosome Structure and the Mechanism of Translation. Cell, 108, 557-572, 2002.

Mears JA, et al. Modeling a Minimal Ribosome Based on Comparative Sequence Analysis. J Mol Biol, 321, 215-234, 2002.

Ban N, Nissen P, Hansen J, Moore PB, Steitz TA. The Complete Atomic Structure of the Large Ribosomal Subunit at 2.4 A Resolution. Science, 289, 905-920, 2000.

Nissen P, Hansen J, Ban N, Moore PB, Steitz TA. The Structural Basis of Ribosome Activity in Peptide Bond Synthesis. Science, 289, 920-930, 2000.

Garrett R. Mechanics of the ribosome. Nature, 400, 811-812, 1999.

Green R and Puglisi JD. The ribosome revealed. Nature Structural Biol, 6(11), 999-1003, 1999.

Rodriguez-Fonseca C, Amils R and Garrett RA. Fine Structure of the Peptidyl Transferase Centre on 23 S-like rRNAs Deduced from Chemical Probing of Antibiotic-Ribosome Complexes. J Mol Biol, 247, 224-235, 1995.