| Plasmids
Sequences .GFP Reassembly
Screen
Mixed Codons Worksheet
List of Publications
Plasmid Sequences  get
pDRAW32 for Windows
Sequence files are text files.
Plasmid Sequences: GFP Reassembly Screen
The sequence files contain detailed
descriptions of how to clone target proteins as in-frame
fusions to the GFP fragments as well as suggested screening
conditions in
E. coli. Also, see below JACS 2005 and Nat.
Methods 2004 articles.
You may request these plasmids by sending
an email to Kathleen
Tatham, the Administrative Assistant of Prof. Regan. Please
include a FedEx number and phone number. Due to the large
volume of requests, we can only send the plasmids by FedEx.
The plasmids are supplied dried on Whatman filter circles,
and must be transformed into E. coli for preparation.
Questions about the system may be directed to Lynne
Regan or Thomas
J. Magliery (presently at Ohio State University). The
plasmids may only be used for not-for-profit academic research.
The underlying technology is covered by U.S. Patent (6,780,599)
assigned to Yale University and exclusively licensed to BioImage
A/S, Soeborg, Denmark. Please contact them for commercial
applications.
Mixed Codons Worksheet by Thomas J. Magliery
Please see Thomas J. Magliery's website
at Ohio State University.
List of Publications
 To
view PDFs, you must install
Adobe Acrobat Reader.
Scanned PDFs are 300 dpi text/line art with color or gray-scale
images attached as necessary. We recommend Acrobat or Acrobat
Reader 5.0 or above and printing with the box next to "Expand
small pages to paper size" unchecked.
2009
Kajander T, Sachs JN, Goldman A, Regan L. 2009, Electrostatic interactions of Hsp-organizing protein tetratricopeptide domains with Hsp70 and Hsp90: computational analysis and protein engineering. J Biol Chem. 284(37): 25364-74. PDF
Champion EA, Kundrat L, Regan L, Baserga, SJ. 2009, A structural model for the HAT domain of Utp6 incorporating bioinformatics and genetics. Protein Eng Des Sel. 22(7): 431-9. PDF
Jackrel ME, Valverde R, Regan L. 2009, Redesign of a protein-peptide interaction: characterization and applications. Protein Sci. 18(4): 762-74. PDF
Yi F, Zhu P, Southall N, Inglese J, Austin CP, Zheng W, Regan L. 2009, An AlphaScreen-Based High-Throughput Screen to Identify Inhibitors of Hsp90-Cochaperone Interaction. J Biomol Screen. 14(3): 273-81. PDF
2008
Yi F, Regan L. 2008, A novel class of small molecule inhibitors of Hsp90. ACS Chem Biol. 3(10): 645-54. PDF
Champion EA, Lane BH, Jackrel ME, Regan L, Baserga SJ. 2008, A direct interaction between the Utp6 half-a-tetratricopeptide repeat domain and a specific peptide in Utp21 is essential for efficient pre-rRNA processing. Mol Cell Biol. 28(21): 6547-56. PDF
Cortajarena AL, Lois G, Sherman E, O'Hern CS, Regan L, Haran G. 2008, Non-random-coil behavior as a consequence of extensive PPII structure in the denatured state. J Mol Biol. 382(1): 203-12. PDF
Grove TZ, Cortajarena AL, Regan L. 2008, Ligand binding by repeat proteins: natural and designed. Curr Opinion Struct Biol. 18(4): 507-15. PDF
Jarymowycz VA, Cortajarena AL, Regan L, Stone MJ. 2008, Comparison of the backbone dynamics of a natural and a consensus designed 3-TPR domain. Biomol NMR. 41 (3): 169-78. PDF
Cortajarena AL, Mochrie SG, Regan L. 2008,
Mapping the energy landscape of repeat proteins using NMR-detected hydrogen exchange. J Mol Biol .379 ( 3 ): 617-26. PDF
Valverde R, Edwards L, Regan L. 2008,
Structure and function of KH domains.
FEBS J . 275 ( 11 ): 2712-26 . PDF
Cortajarena AL, Yi F, Regan L. 2008,
Designed TPR modules as novel anticancer agents,
ACS Chem Biol . 3 ( 3 ): 161-6 . PDF
Dalal S, Canet D, Kaiser SE, Dobson CM, Regan L., 2008 Conservation of mechanism, variation of rate: folding kinetics of three homologous four-helix bundle proteins. Protein Eng Des Sel .21 ( 3 ): 197-206 .PDF Nuland NA van, Dobson CM, Regan L. (2008)
Characterization of folding the four-helix bundle protein Rop by real-time NMR, Prot Eng Des Select 21 (3), 165-70. PDF
2007
Valverde R, Pozdnyakova I, Kajander T,Venkatraman J, Regan
L. 2007 Fragile X Mental Retardation Syndrome: Structure of
the KH1-KH2 Domains of Fragile X Mental Retardation Protein,
Structure, 15:1090-1098. PDF
Kajander T, Cortajarena AL,Monchire S, Regan L. 2007.Structure
and stability of designed TPR protein superhelices: unusual
crystal packing and implications for natural TPR proteins,
Acta Crystallographica Section D, Biological Crystallography,
D63: 800-811. PDF
Cheng, CY, Jarymowycz, VA, Cortajarena, AL, Regan,L,and Stone,
MJ. 2006.Repeat Motions and Backbone Flexibility in Designed
Proteins with Different
Numbers of Identical Consensus Tetratricopeptide Repeats.
Biochemistry, 45: 12175-12183. PDF
2006
Kajander T, Cortajarena AL,Regan L. 2006. Consensus Design
as a Tool for Engineering Repeat Proteins. Methods in
Molecular Biology. 340, page 151-305.
PDF
Cortajarena, AL, Regan L. 2006.
Ligand binding by TPR domains
Protein Science 15:1193- 1198.
PDF
Magliery TJ, Regan L. 2006.
Reassembled GFP: detecting protein-protein interactions and protein expression patterns.
Methods Biochem Anal. 47:391-405.
PubMed
2005
Magliery TJ, Regan L. 2005.
Sequence variation in ligand binding sites in proteins.
BMC Bioinformatics. 6:240.
PDF
Wang J, Gulich S, Bradford C, Ramirez-Alvarado M, Regan L. 2005.
A twisted four-sheeted model for an amyloid fibril.
Structure. 13(9):1279-88.
PDF
Main ER, Lowe AR, Mochrie SG, Jackson SE, Regan L. 2005.
A recurring theme in protein engineering: the design, stability and folding of repeat proteins.
Curr Opin Struct Biol. 5(4):464-71
PDF
Kajander T, Cortajarena AL, Main ER, Mochrie SG, Regan L. 2005.
A new folding paradigm for repeat proteins. J Am Chem Soc. 127(29):10188-90
PDF• Supplementary
Information•
Main ER, Stott K, Jackson SE, Regan L. 2005. Local and long-range
stability in tandemly arrayed tetratricopeptide repeats. Proc
Natl Acad Sci USA 102: 5721. PDF
Pozdnyakova I, Regan L. 2005. New insights into Fragile
X syndrome. Relating genotype to phenotype at the molecular
level. FEBS J 272: 872. PDF
Wilson CG, Kajander T, Regan L. 2005. The crystal structure
of NlpI. A prokaryotic tetratricopeptide repeat protein with
a globular fold. FEBS J 272: 166. PDF
Magliery TJ, Wilson CGM, Pan W, Mishler D, Ghosh I, Hamilton
AD, Regan L. 2005. Detecting protein-protein interactions
with a green fluorescent protein fragment reassembly trap:
scope and mechanism. J Am Chem Soc 127: 146 . PDF
• Supplementary
Information
2004
Wilson CGM, Magliery TJ, Regan L. 2004. Detecting protein-protein
interactions with GFP-fragment reassembly. Nat Methods 1:
255.
PDF • Supplementary
Figure • Supplementary
Note
Magliery TJ, Regan L. 2004. Beyond consensus:
statistical free energies reveal hidden interactions in the design of a TPR
motif. J Mol Biol 343: 731. PDF • Supplementary
Information
Cortajarena AL, Kajander T, Pan W, Cocco M, Regan L. 2004.
Protein design to understand peptide ligand recognition by
tetratricopeptide repeat proteins. Prot Eng Des Select
17: 399. PDF
 Magliery
TJ, Regan L. 2004. Library approaches to biophysical problems
(editorial overview). Eur J Biochem 271:
1593. PDF
Magliery TJ, Regan L. 2004. Combinatorial approaches to
protein stability and structure. Eur J Biochem 271:
1595. PDF
Also, click cover to see accompanying reviews by
Yawen Bai, David Baker, Andrea Cochran and Donald Hilvert.
Magliery TJ, Regan L. 2004. A cell-based
screen for function of the four-helix bundle protein Rop:
a new tool for combinatorial experiments in biophysics. Protein
Eng Des Select
17: 77. PDF
2003
D'Andrea L, Regan L. 2003. TPR proteins: the versatile helix.
Trends Biochem Sci 28: 655. PDF
Mayer KL, Earley MR, Gupta S, Pichumani K, Regan L, Stone
MJ. 2003. Covariation in backbone motion throughout a small
protein domain. Nat Struct Biol 10: 962. PDF
Main ER, Jackson SE, Regan L. 2003. The folding and design
of repeat proteins: reaching a consensus. Curr Opin Struct
Biol 13: 482. PDF
Regan L, Jackson SE. 2003. Engineering and design. Protein
design: theory and practice. Curr Opin Struct Biol
13: 479. PDF
Khurana R, Ionescu-Zanetti C, Pope M, Li J, Nielson L,
Ramirez-Alvarado M, Regan L, Fink AL, Carter SA . 2003.
A general model for amyloid fibril assembly based on morphological
studies using atomic force microscopy. Biophys J 85:
1135. PDF
Main ERG, Xiong Y, Cocco MJ, D'Andrea L, Regan L. 2003. Design
of stable α-helical arrays from an idealized TPR motif.
Structure 11: 497. PDF
Regan L. 2003. Molten globules move into action. Proc
Natl Acad Sci USA 100: 3553. PDF
Ramirez-Alvarado M, Cocco MJ, Regan L. 2003. Mutations in
the B1 domain of protein G that delay the onset of amyloid
fibril formation in vitro. Protein Sci 12: 567. PDF
2002
Ramirez-Alvarado M, Regan L. 2002. Does the location of a
mutation determine the ability to form amyloid fibrils? J
Mol Biol 323: 17. PDF
2001
Marino SF, Shechner D, Regan L. 2001. 'Morphs' (MRFs): metal-reversible
folding domains for differential IgG binding. Chem Biol
8: 1221. PDF
Bishop B, Koay DC, Sartorelli AC, Regan L. 2001. Reengineering
granulocyte colony-stimulating factor for enhanced stability.
J Biol Chem 276: 33465. PDF
Stone MJ, Gupta S, Snyder N, Regan L. 2001. Comparison of
protein backbone entropy and beta sheet stability: NMR-derived
dynamics of protein G B1 domain mutants. J Am Chem Soc
123: 185. PDF
Regan L. 2001. Engineering and design 2001: a design odyssey.
Curr Op Struc Biol 11: 449. PDF
Regan L. 2001. Current genomic research: the proteins have
it. National Academy of Engineering: Sixth Annual Symposium
on Frontiers of Engineering. Washington, DC: National
Academy Press. pp 54-61. PDF
2000
Willis MA, Bishop B, Regan L, Brunger AT. 2000. Dramatic
structural and thermodynamic consequences of repacking a protein's
hydrophobic core. Structure Fold Des 8: 1319. PDF
Ramirez-Alvarado M, Merkel JS, Regan L. 2000. A systematic
exploration of the influence of the protein stability on amyloid
fibril formation in vitro. Proc Natl Acad Sci USA
97: 8979.
PDF
Merkel JS, Regan L. 2000. Modulating protein folding rates
in vivo and in vitro by side-chain interactions between the
parallel beta strands of green fluorescent protein. J
Biol Chem 275: 29200. PDF
Dalal S, Regan L. 2000. Understanding the sequence determinants
of conformational switching using protein design. Protein
Sci 9: 1651. PDF
Seewald MJ, Pichumani K, Stowell C, Tibbals BV, Regan L,
Stone MJ. 2000. The role of backbone conformational heat capacity
in protein stability: temperature dependent dynamics of the
B1 domain of Streptococcal protein G. Protein Sci
9: 1177. PDF
Balasubramanian S, Schneider T, Gerstein M, Regan L. 2000.
Proteomics of Mycoplasma genitalium: identification and characterization
of unannotated and atypical proteins in a small model genome.
Nucleic Acids Res 28: 3075. PDF
Ghosh I, Hamilton AD, Regan L. 2000. Antiparallel leucine
zipper-directed protein reassembly: application to the green
fluorscent protein. J Am Chem Soc 122: 5658. PDF
• Supporting
Information
Lurio LB, Lumma D, Sandy AR, Borthwick MA, Falus P, Mochrie
SGJ, Pelletier J-F, Sutton M, Regan L, Malik A, Stephenson
GB. 2000. Absence of scaling for the intermediate scattering
function of a hard-sphere suspension: static and dynamic X-ray
scattering from concentrated polystyrene latex spheres. Phys
Rev Lett 84: 785. PDF
Regan L. 1999. Protein redesign. Curr Opin Struct Biol
9: 494. PDF
Nagi AD, Anderson KS, Regan L. 1999. Using loop length variants
to dissect the folding pathway of a four-helix-bundle protein.
J Mol Biol 286: 257. PDF
Merkel JS, Sturtevant JM, Regan L. 1999. Sidechain interactions
in parallel beta sheets: the energetics of cross-strand pairings.
Structure Fold Des 7: 1333. PDF
Marino SF, Regan L. 1999. Secondary ligands enhance affinity
at a designed metal-binding site. Chem Biol 6: 649.
PDF
Regan L, Wells J. 1998. Engineering and design: recent advances
in molecular design. Curr Op Struc Biol 8: 441. PDF
Klemba MW, Munson M, Regan L. 1998. De novo design of protein
structure and function. Proteins, Analysis and Design.
ed. Angeletti RH. Academic Press.
Regan L. 1998. Proteins to order? Structure 6: 1.
PDF
Prodromou C, Siligardi G, O'Brien R, Woolfson DN, Regan L,
Panaretou B, Ladbury JE, Piper PW, Pearl LH. 1999. Regulation
of Hsp90 ATPase activity by tetratricopeptiderepeat(TPR)-domain
co-chaperones. EMBO J 18: 754. PDF
Ma Y, Cunningham ME, Wang, X, Ghosh I, Regan L, Longely BJ.
1999. Inhibition of spontaneous receptor phosphorylation by
residues in a putatitve alpha-helix in the KIT intracellular
juxtamembrane region. J Biol Chem 274 13399. PDF
Merkel JS, Regan L. 1998. Aromatic rescue of glycine in beta
sheets. Fold Des 3: 449. PDF
Farinas E, Regan L. 1998. The de novo design of a rubredoxin-like
Fe site. Protein Sci 7: 1939. PDF
Smith CK, Regan L. 1997. Construction and design of beta-sheets.
Acc Chem Res 30: 153. PDF
Regan L. 1997. Engineering metal-binding site in proteins.
Advances in Mol Cell Biol. ed. Allewell N, Woodward
C. 22A&B. Elsevier.
Regan L. 1997. Helix is a helix is a helix? Proc Natl
Acad Sci USA 94: 2796. PDF
Nagi AD, Regan L. 1997. An inverse correlation between loop
length and stability in a four-helix-bundle protein. Fold
Des 2: 67. PDF
Munson M, Anderson KS, Regan L. 1997. Speeding up protein
folding: mutations that increase the rate at which Rop folds
and unfolds by over four orders of magnitude. Fold Des
2: 77. PDF
Dalal S, Balasubramanian S, Regan L. 1997. Transmuting alpha
helices and beta sheets. Fold Des 2: R71. PDF
Dalal S, Balasubramanian S, Regan L. 1997. Protein alchemy:
changing beta-sheet into alpha-helix. Nat Struct Biol
4: 548. PDF
(scanned)
Smith CK, Bu Z, Anderson KS, Sturtevant JM, Engelman DM,
Regan L. 1996. Surface point mutations that significantly
alter the structure and stability of a protein's denatured
state. Protein Sci 5: 2009. PDF
Predki PF, Agrawal V, Brunger AT, Regan L. 1996. Amino-acid
substitutions in a surface turn modulate protein stability.
Nat Struct Biol 3: 54. PDF
(scanned)
Munson M, Balasubramanian S, Fleming KG, Nagi AD, O'Brien
R, et al. 1996. What makes a protein a protein? Hydrophobic
core designs that specify stability and structural properties.
Protein Sci 5: 1584. PDF
Smith CK, Munson M, Regan L. 1995. Studying alpha-helix and
beta-sheet formation in small proteins. Techniques in
Protein Chemistry VI: 323.
Smith CK, Regan L. 1995. Guidelines for protein design: the
energetics of beta sheet side chain interactions. Science
270: 980. PDF
(scanned)
Regan L. 1995. Protein design: novel metal-binding sites.
Trends Biochem Sci 20: 280. PDF
Predki PF, Regan L. 1995. Redesigning the topology of a four-helix-bundle
protein: monomeric Rop. Biochemistry 34: 9834. PDF
Predki PF, Nayak LM, Gottlieb MB, Regan L. 1995. Dissecting
RNA-protein interactions: RNA-RNA recognition by Rop. Cell
80: 41. PDF
(scanned)
Klemba M, Regan L. 1995. Characterization of metal binding
by a designed protein: single ligand substitutions at a tetrahedral
Cys2His2 site. Biochemistry 34: 10094. PDF
Klemba M, Gardner KH, Marino S, Clarke ND, Regan L. 1995.
Novel metal-binding proteins by design. Nat Struct Biol
2: 368. PDF
(scanned)
Smith CK, Withka JM, Regan L. 1994. A thermodynamic scale
for the beta-sheet forming tendencies of the amino acids.
Biochemistry 33: 5510. PDF
Regan L, Rockwell A, Wasserman Z, DeGrado W. 1994. Disulfide
crosslinks to probe the structure and flexibility of a designed
four-helix bundle protein. Protein Sci 3: 2419. PDF
Regan L. 1994. Protein structure. Born to be beta. Curr
Biol 4: 656. PDF
(scanned)
Munson M, Predki PF, Regan L. 1994.
ColE1-compatible vectors for high-level expression of cloned
DNAs from the T7 promoter. Gene 144: 59. PDF
(scanned)
Munson M, O'Brien R, Sturtevant JM, Regan L. 1994. Redesigning
the hydrophobic core of a four-helix-bundle protein. Protein
Sci 3: 2015. PDF
Regan L. 1993. What determines where alpha-helices begin
and end? Proc Natl Acad Sci USA 90: 10907. PDF
Regan L. 1993. The design of metal-binding sites in proteins.
Annu Rev Biophys Biomol Struct 22: 257. PDF
(scanned)
Marqusee S, Regan L. 1991. Deconstructing protein structure.
Curr Biol 1: 207. PDF
(scanned)
Regan L. 1991. Protein design. Curr Opin Biotechnol
2: 544. PubMed
Regan L, Clarke N. 1990. A tetrahedral Zn(II)-binding site
introduced into a designed protein. Biochemistry
29: 10878. PDF
Regan L, Ho SP, Wasserman Z, Degrado WF. 1990. Helical proteins.
de novo design. Protein Folding: Deciphering the Second
Half of the Genetic Code. ed. Gierasch LM, King J. Washington,
D.C.: AAAS.
Regan L, DeGrado WF. 1988. Characterization of a helical
protein designed from first principles. Science 241:
976. PDF
Regan L, Buxbaum L, Hill K, Schimmel P. 1988. Rationale for
engineering an enzyme by introducing a mutation that compensates
for a deletion. J Biol Chem 263: 18598. PDF
Frederick CA, Wang AH, Rich A, Regan L, Schimmel P. 1988.
Crystallization of a small fragment of an aminoacyl tRNA synthetase.
J Mol Biol 203: 521. PubMed
Regan L, Schimmel P. 1987. Selection of aminoacyl tRNA synthetases
with enhanced catalytic activity. UCLA Symp Mol Cell Biol
69: 293.
Regan L, Bowie J, Schimmel P. 1987. Polypeptide sequences
essential for RNA recognition by an enzyme. Science
235: 1651. PDF
DeGrado WF, Regan L, Ho SP. 1987. The design of a four-helix
bundle protein. Cold Spring Harb Symp Quant Biol 52:
521. PubMed
DeGrado WF, Ho S, Wasserman Z, Regan L. 1987. The design
and characterization of four-helix-bundle proteins. UCLA
Symp Mol Cell Biol 69: 429.
Regan L, Dignam JD, Schimmel P. 1986. A bacterial and silkworm
aminoacyl-tRNA synthetase have a common epitope which maps
to the catalytic domain of each. J Biol Chem 261:
5241. PDF
Jasin M, Regan L, Schimmel P. 1985. Two mutations in the
dispensable part of alanine tRNA synthetase which affect the
catalytic activity. J Biol Chem 260: 2226. PDF
Schimmel P, Jasin M, Regan L. 1984. Size polymorphism and
the structure of aminoacyl-tRNA synthetases. Fed Proc
43: 2987. PubMed
Jasin M, Regan L, Schimmel P. 1984. Dispensable pieces of
an aminoacyl tRNA synthetase which activate the catalytic
site. Cell 36: 1089. PubMed
Jasin M, Regan L, Schimmel P. 1983. Modular arrangement of
functional domains along the sequence of an aminoacyl tRNA
synthetase. Nature 306: 441. PubMed
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