List of Publications
2012
Schlingman, D.J.*, Mack, A.H*.,Ilagan, R.P., Mochrie, S.G.J. and Regan, L. 2012, Kinetics and thermodynamics of phenotype: Unwinding and rewinding the nucleosome. Journal of Molecular Biology, 423: PDF
Mack, A.H., Schlingman, D.J., Regan, L., and Mochrie, S.G.J. 2012, Practical Axial Optical Trapping. Review of Scientific Instruments, 83:10 PDF
Grove, T.Z., and Regan, L. 2012, New materials from proteins and peptides. Current Opinion in Structural Biology, 22: 451-456. PDF
Zhou, A.Q., O'Hern, C.S. and Regan, L. 2012, The Power of Hard-Sphere Models: Explaining Side-Chain Dihedral Angle Distributions of Thr and Val. Biophysical Journal, 102: 2345-2352. PDF
Liberles, D.A., Teichmann, S.A., Bahar, I., Bastolla, U., Bloom, J., Bornberg-Bauer, E., Colwell, L.J., de Koning, A.P.J., Dokholyan, N.V., Echave, J. et al. 2012, The interface of protein structure, protein biophysics, and molecular evolution. Protein science : a publication of the Protein Society, 21: 769-785. PDF
Grove, T.Z., Forster, J., Pimienta, G., Dufresne, E. and Regan, L. 2012, A modular approach to the design of protein-based smart gels. Biopolymers, 97: 508-517. PDF
De Rosa, L., Cortajarena, A.L., Romanelli, A., Regan, L. and D'Andrea, L.D. 2012, Site-specific protein double labeling by expressed protein ligation: applications to repeat proteins. Organic & Biomolecular Chemistry, 10: 273-280. PDF
2011
Zhou, A.Q., O'Hern, C.S. and Regan, L. 2011, Revisiting the Ramachandran plot from a new angle. Protein Science, 20: 1166-1171. PDF
Zhou, A.Q., O'Hern, C.S. and Regan, L. 2011, Reply to: Comment on "Revisiting the Ramachandran plot from a new angle". Protein Science, 20: 1774-1774.
Schlingman, D.J., Mack, A.H., Mochrie, S.G.J. and Regan, L. 2011, A new method for the covalent attachment of DNA to a surface for single-molecule studies. Colloids and Surfaces B-Biointerfaces, 83: 91-95. PDF
Pimienta, G., Herbert, K.M. and Regan, L. 2011, A Compound That Inhibits the HOP-Hsp90 Complex Formation and Has Unique Killing Effects in Breast Cancer Cell Lines. Molecular Pharmaceutics, 8: 2252-2261. PDF
Cortajarena, A.L. and Regan, L. 2011, Calorimetric study of a series of designed repeat proteins: Modular structure and modular folding. Protein Science, 20: 336-340. PDF
Cortajarena, A.L., Mochrie, S.G.J. and Regan, L. 2011, Modulating repeat protein stability: The effect of individual helix stability on the collective behavior of the ensemble. Protein Science, 20: 1042-1047. PDF
2010
Yi, F., Doudevski, I. and Regan, L. 2010, HOP is a monomer: Investigation of the oligomeric state of the co-chaperone HOP. Protein Science, 19: 19-25. PDF
Kundrat, L. and Regan, L. 2010, Identification of Residues on Hsp70 and Hsp90 Ubiquitinated by the Cochaperone CHIP. Journal of Molecular Biology, 395:587-594. PDF
Kundrat, L. and Regan, L. 2010, Balance between Folding and Degradation for Hsp90-Dependent Client Proteins: A Key Role for CHIP. Biochemistry, 49: 7428-7438. PDF
Jackrel, M.E., Cortajarena, A.L., Liu, T.Y. and Regan, L. 2010, Screening Libraries To Identify Proteins with Desired Binding Activities Using a Split-GFP Reassembly Assay. Acs Chemical Biology, 5: 553-562. PDF
Ilagan, R.P., Rhoades, E., Gruber, D.F., Kao, H.-T., Pieribone, V.A. and Regan, L. 2010, A new bright green-emitting fluorescent protein - engineered monomeric and dimeric forms. Febs Journal, 277: 1967-1978. PDF
Grove, T.Z., Osuji, C.O., Forster, J.D., Dufresne, E.R. and Regan, L. 2010, Stimuli-Responsive Smart Gels Realized via Modular Protein Design. Journal of the American Chemical Society, 132: 14024-14026. PDF
Grove, T.Z., Hands, M. and Regan, L. 2010, Creating novel proteins by combining design and selection. Protein Engineering Design & Selection , 23: 449-455. PDF
Cortajarena, A.L., Wang, J. and Regan, L. 2010, Crystal structure of a designed tetratricopeptide repeat module in complex with its peptide ligand. Febs Journal, 277: 1058-1066. PDF
Cortajarena, A.L., Liu, T.Y., Hochstrasser, M. and Regan, L. 2010, Designed Proteins To Modulate Cellular Networks. Acs Chemical Biology, 5: 545-552. PDF
Clarke, J. and Regan, L. 2010, Protein engineering and design: from first principles to new technologies. Current Opinion in Structural Biology, 20: 480-481. PDF
2009
Kajander T, Sachs JN, Goldman A, Regan L. 2009, Electrostatic interactions of Hsp-organizing protein tetratricopeptide domains with Hsp70 and Hsp90: computational analysis and protein engineering. J Biol Chem. 284(37): 25364-74. PDF
Champion EA, Kundrat L, Regan L, Baserga, SJ. 2009, A structural model for the HAT domain of Utp6 incorporating bioinformatics and genetics. Protein Eng Des Sel. 22(7): 431-9. PDF
Jackrel ME, Valverde R, Regan L. 2009, Redesign of a protein-peptide interaction: characterization and applications. Protein Sci. 18(4): 762-74. PDF
Yi F, Zhu P, Southall N, Inglese J, Austin CP, Zheng W, Regan L. 2009, An AlphaScreen-Based High-Throughput Screen to Identify Inhibitors of Hsp90-Cochaperone Interaction. J Biomol Screen. 14(3): 273-81. PDF
2008
Yi F, Regan L. 2008, A novel class of small molecule inhibitors of Hsp90. ACS Chem Biol. 3(10): 645-54. PDF
Champion EA, Lane BH, Jackrel ME, Regan L, Baserga SJ. 2008, A direct interaction between the Utp6 half-a-tetratricopeptide repeat domain and a specific peptide in Utp21 is essential for efficient pre-rRNA processing. Mol Cell Biol. 28(21): 6547-56. PDF
Cortajarena AL, Lois G, Sherman E, O'Hern CS, Regan L, Haran G. 2008, Non-random-coil behavior as a consequence of extensive PPII structure in the denatured state. J Mol Biol. 382(1): 203-12. PDF
Grove TZ, Cortajarena AL, Regan L. 2008, Ligand binding by repeat proteins: natural and designed. Curr Opinion Struct Biol. 18(4): 507-15. PDF
Jarymowycz VA, Cortajarena AL, Regan L, Stone MJ. 2008, Comparison of the backbone dynamics of a natural and a consensus designed 3-TPR domain. Biomol NMR. 41 (3): 169-78. PDF
Cortajarena AL, Mochrie SG, Regan L. 2008, Mapping the energy landscape of repeat proteins using NMR-detected hydrogen exchange. J Mol Biol .379 ( 3 ): 617-26. PDF
Valverde R, Edwards L, Regan L. 2008, Structure and function of KH domains. FEBS J . 275 ( 11 ): 2712-26 . PDF
Cortajarena AL, Yi F, Regan L. 2008, Designed TPR modules as novel anticancer agents, ACS Chem Biol . 3 ( 3 ): 161-6 . PDF
Dalal S, Canet D, Kaiser SE, Dobson CM, Regan L., 2008 Conservation of mechanism, variation of rate: folding kinetics of three homologous four-helix bundle proteins. Protein Eng Des Sel .21 ( 3 ): 197-206 .PDF
Nuland NA van, Dobson CM, Regan L. (2008) Characterization of folding the four-helix bundle protein Rop by real-time NMR, Prot Eng Des Select 21 (3), 165-70. PDF
2007
Valverde R, Pozdnyakova I, Kajander T,Venkatraman J, Regan L. 2007 Fragile X Mental Retardation Syndrome: Structure of the KH1-KH2 Domains of Fragile X Mental Retardation Protein, Structure, 15:1090-1098. PDF
Kajander T, Cortajarena AL,Monchire S, Regan L. 2007.Structure and stability of designed TPR protein superhelices: unusual crystal packing and implications for natural TPR proteins, Acta Crystallographica Section D, Biological Crystallography, D63: 800-811. PDF
Cheng, CY, Jarymowycz, VA, Cortajarena, AL, Regan,L,and Stone,
MJ. 2006.Repeat Motions and Backbone Flexibility in Designed
Proteins with Different
Numbers of Identical Consensus Tetratricopeptide Repeats.
Biochemistry, 45: 12175-12183. PDF
2006
Kajander T, Cortajarena AL,Regan L. 2006. Consensus Design as a Tool for Engineering Repeat Proteins. Methods in Molecular Biology. 340, page 151-305. PDF
Cortajarena, AL, Regan L. 2006. Ligand binding by TPR domains Protein Science 15:1193- 1198. PDF
Magliery TJ, Regan L. 2006. Reassembled GFP: detecting protein-protein interactions and protein expression patterns. Methods Biochem Anal. 47:391-405. PubMed
2005
Magliery TJ, Regan L. 2005. Sequence variation in ligand binding sites in proteins. BMC Bioinformatics. 6:240. PDF
Wang J, Gulich S, Bradford C, Ramirez-Alvarado M, Regan L. 2005. A twisted four-sheeted model for an amyloid fibril. Structure. 13(9):1279-88. PDF
Main ER, Lowe AR, Mochrie SG, Jackson SE, Regan L. 2005. A recurring theme in protein engineering: the design, stability and folding of repeat proteins. Curr Opin Struct Biol. 5(4):464-71 PDF
Kajander T, Cortajarena AL, Main ER, Mochrie SG, Regan L. 2005. A new folding paradigm for repeat proteins. J Am Chem Soc. 127(29):10188-90 PDF• Supplementary Information•
Main ER, Stott K, Jackson SE, Regan L. 2005. Local and long-range stability in tandemly arrayed tetratricopeptide repeats. Proc Natl Acad Sci USA 102: 5721. PDF
Pozdnyakova I, Regan L. 2005. New insights into Fragile X syndrome. Relating genotype to phenotype at the molecular level. FEBS J 272: 872. PDF
Wilson CG, Kajander T, Regan L. 2005. The crystal structure of NlpI. A prokaryotic tetratricopeptide repeat protein with a globular fold. FEBS J 272: 166. PDF
Magliery TJ, Wilson CGM, Pan W, Mishler D, Ghosh I, Hamilton AD, Regan L. 2005. Detecting protein-protein interactions with a green fluorescent protein fragment reassembly trap: scope and mechanism. J Am Chem Soc 127: 146 . PDF • Supplementary Information
2004
Wilson CGM, Magliery TJ, Regan L. 2004. Detecting protein-protein interactions with GFP-fragment reassembly. Nat Methods 1: 255. PDF • Supplementary Figure • Supplementary Note
Magliery TJ, Regan L. 2004. Beyond consensus: statistical free energies reveal hidden interactions in the design of a TPR motif. J Mol Biol 343: 731. PDF • Supplementary Information
Cortajarena AL, Kajander T, Pan W, Cocco M, Regan L. 2004. Protein design to understand peptide ligand recognition by tetratricopeptide repeat proteins. Prot Eng Des Select 17: 399. PDF
Magliery
TJ, Regan L. 2004. Library approaches to biophysical problems
(editorial overview). Eur J Biochem 271:
1593. PDF
Magliery TJ, Regan L. 2004. Combinatorial approaches to protein stability and structure. Eur J Biochem 271: 1595. PDF
Also, click cover to see accompanying reviews by Yawen Bai, David Baker, Andrea Cochran and Donald Hilvert.
Magliery TJ, Regan L. 2004. A cell-based screen for function of the four-helix bundle protein Rop: a new tool for combinatorial experiments in biophysics. Protein Eng Des Select 17: 77. PDF
2003
D'Andrea L, Regan L. 2003. TPR proteins: the versatile helix. Trends Biochem Sci 28: 655. PDF
Mayer KL, Earley MR, Gupta S, Pichumani K, Regan L, Stone MJ. 2003. Covariation in backbone motion throughout a small protein domain. Nat Struct Biol 10: 962. PDF
Main ER, Jackson SE, Regan L. 2003. The folding and design of repeat proteins: reaching a consensus. Curr Opin Struct Biol 13: 482. PDF
Regan L, Jackson SE. 2003. Engineering and design. Protein design: theory and practice. Curr Opin Struct Biol 13: 479. PDF
Khurana R, Ionescu-Zanetti C, Pope M, Li J, Nielson L, Ramirez-Alvarado M, Regan L, Fink AL, Carter SA . 2003. A general model for amyloid fibril assembly based on morphological studies using atomic force microscopy. Biophys J 85: 1135. PDF
Main ERG, Xiong Y, Cocco MJ, D'Andrea L, Regan L. 2003. Design of stable α-helical arrays from an idealized TPR motif. Structure 11: 497. PDF
Regan L. 2003. Molten globules move into action. Proc Natl Acad Sci USA 100: 3553. PDF
Ramirez-Alvarado M, Cocco MJ, Regan L. 2003. Mutations in the B1 domain of protein G that delay the onset of amyloid fibril formation in vitro. Protein Sci 12: 567. PDF
2002
Ramirez-Alvarado M, Regan L. 2002. Does the location of a mutation determine the ability to form amyloid fibrils? J Mol Biol 323: 17. PDF
2001
Marino SF, Shechner D, Regan L. 2001. 'Morphs' (MRFs): metal-reversible folding domains for differential IgG binding. Chem Biol 8: 1221. PDF
Bishop B, Koay DC, Sartorelli AC, Regan L. 2001. Reengineering granulocyte colony-stimulating factor for enhanced stability. J Biol Chem 276: 33465. PDF
Stone MJ, Gupta S, Snyder N, Regan L. 2001. Comparison of protein backbone entropy and beta sheet stability: NMR-derived dynamics of protein G B1 domain mutants. J Am Chem Soc 123: 185. PDF
Regan L. 2001. Engineering and design 2001: a design odyssey. Curr Op Struc Biol 11: 449. PDF
Regan L. 2001. Current genomic research: the proteins have it. National Academy of Engineering: Sixth Annual Symposium on Frontiers of Engineering. Washington, DC: National Academy Press. pp 54-61. PDF
2000
Willis MA, Bishop B, Regan L, Brunger AT. 2000. Dramatic structural and thermodynamic consequences of repacking a protein's hydrophobic core. Structure Fold Des 8: 1319. PDF
Ramirez-Alvarado M, Merkel JS, Regan L. 2000. A systematic exploration of the influence of the protein stability on amyloid fibril formation in vitro. Proc Natl Acad Sci USA 97: 8979. PDF
Merkel JS, Regan L. 2000. Modulating protein folding rates in vivo and in vitro by side-chain interactions between the parallel beta strands of green fluorescent protein. J Biol Chem 275: 29200. PDF
Dalal S, Regan L. 2000. Understanding the sequence determinants of conformational switching using protein design. Protein Sci 9: 1651. PDF
Seewald MJ, Pichumani K, Stowell C, Tibbals BV, Regan L, Stone MJ. 2000. The role of backbone conformational heat capacity in protein stability: temperature dependent dynamics of the B1 domain of Streptococcal protein G. Protein Sci 9: 1177. PDF
Balasubramanian S, Schneider T, Gerstein M, Regan L. 2000. Proteomics of Mycoplasma genitalium: identification and characterization of unannotated and atypical proteins in a small model genome. Nucleic Acids Res 28: 3075. PDF
Ghosh I, Hamilton AD, Regan L. 2000. Antiparallel leucine zipper-directed protein reassembly: application to the green fluorscent protein. J Am Chem Soc 122: 5658. PDF • Supporting Information
Lurio LB, Lumma D, Sandy AR, Borthwick MA, Falus P, Mochrie SGJ, Pelletier J-F, Sutton M, Regan L, Malik A, Stephenson GB. 2000. Absence of scaling for the intermediate scattering function of a hard-sphere suspension: static and dynamic X-ray scattering from concentrated polystyrene latex spheres. Phys Rev Lett 84: 785. PDF
Regan L. 1999. Protein redesign. Curr Opin Struct Biol 9: 494. PDF
Nagi AD, Anderson KS, Regan L. 1999. Using loop length variants to dissect the folding pathway of a four-helix-bundle protein. J Mol Biol 286: 257. PDF
Merkel JS, Sturtevant JM, Regan L. 1999. Sidechain interactions in parallel beta sheets: the energetics of cross-strand pairings. Structure Fold Des 7: 1333. PDF
Marino SF, Regan L. 1999. Secondary ligands enhance affinity at a designed metal-binding site. Chem Biol 6: 649. PDF
Regan L, Wells J. 1998. Engineering and design: recent advances in molecular design. Curr Op Struc Biol 8: 441. PDF
Klemba MW, Munson M, Regan L. 1998. De novo design of protein structure and function. Proteins, Analysis and Design. ed. Angeletti RH. Academic Press.
Regan L. 1998. Proteins to order? Structure 6: 1. PDF
Prodromou C, Siligardi G, O'Brien R, Woolfson DN, Regan L, Panaretou B, Ladbury JE, Piper PW, Pearl LH. 1999. Regulation of Hsp90 ATPase activity by tetratricopeptiderepeat(TPR)-domain co-chaperones. EMBO J 18: 754. PDF
Ma Y, Cunningham ME, Wang, X, Ghosh I, Regan L, Longely BJ. 1999. Inhibition of spontaneous receptor phosphorylation by residues in a putatitve alpha-helix in the KIT intracellular juxtamembrane region. J Biol Chem 274 13399. PDF
Merkel JS, Regan L. 1998. Aromatic rescue of glycine in beta sheets. Fold Des 3: 449. PDF
Farinas E, Regan L. 1998. The de novo design of a rubredoxin-like Fe site. Protein Sci 7: 1939. PDF
Smith CK, Regan L. 1997. Construction and design of beta-sheets. Acc Chem Res 30: 153. PDF
Regan L. 1997. Engineering metal-binding site in proteins. Advances in Mol Cell Biol. ed. Allewell N, Woodward C. 22A&B. Elsevier.
Regan L. 1997. Helix is a helix is a helix? Proc Natl Acad Sci USA 94: 2796. PDF
Nagi AD, Regan L. 1997. An inverse correlation between loop length and stability in a four-helix-bundle protein. Fold Des 2: 67. PDF
Munson M, Anderson KS, Regan L. 1997. Speeding up protein folding: mutations that increase the rate at which Rop folds and unfolds by over four orders of magnitude. Fold Des 2: 77. PDF
Dalal S, Balasubramanian S, Regan L. 1997. Transmuting alpha helices and beta sheets. Fold Des 2: R71. PDF
Dalal S, Balasubramanian S, Regan L. 1997. Protein alchemy: changing beta-sheet into alpha-helix. Nat Struct Biol 4: 548. PDF (scanned)
Smith CK, Bu Z, Anderson KS, Sturtevant JM, Engelman DM, Regan L. 1996. Surface point mutations that significantly alter the structure and stability of a protein's denatured state. Protein Sci 5: 2009. PDF
Predki PF, Agrawal V, Brunger AT, Regan L. 1996. Amino-acid substitutions in a surface turn modulate protein stability. Nat Struct Biol 3: 54. PDF (scanned)
Munson M, Balasubramanian S, Fleming KG, Nagi AD, O'Brien R, et al. 1996. What makes a protein a protein? Hydrophobic core designs that specify stability and structural properties. Protein Sci 5: 1584. PDF
Smith CK, Munson M, Regan L. 1995. Studying alpha-helix and beta-sheet formation in small proteins. Techniques in Protein Chemistry VI: 323.
Smith CK, Regan L. 1995. Guidelines for protein design: the energetics of beta sheet side chain interactions. Science 270: 980. PDF (scanned)
Regan L. 1995. Protein design: novel metal-binding sites. Trends Biochem Sci 20: 280. PDF
Predki PF, Regan L. 1995. Redesigning the topology of a four-helix-bundle protein: monomeric Rop. Biochemistry 34: 9834. PDF
Predki PF, Nayak LM, Gottlieb MB, Regan L. 1995. Dissecting RNA-protein interactions: RNA-RNA recognition by Rop. Cell 80: 41. PDF (scanned)
Klemba M, Regan L. 1995. Characterization of metal binding by a designed protein: single ligand substitutions at a tetrahedral Cys2His2 site. Biochemistry 34: 10094. PDF
Klemba M, Gardner KH, Marino S, Clarke ND, Regan L. 1995. Novel metal-binding proteins by design. Nat Struct Biol 2: 368. PDF (scanned)
Smith CK, Withka JM, Regan L. 1994. A thermodynamic scale for the beta-sheet forming tendencies of the amino acids. Biochemistry 33: 5510. PDF
Regan L, Rockwell A, Wasserman Z, DeGrado W. 1994. Disulfide crosslinks to probe the structure and flexibility of a designed four-helix bundle protein. Protein Sci 3: 2419. PDF
Regan L. 1994. Protein structure. Born to be beta. Curr Biol 4: 656. PDF (scanned)
Munson M, Predki PF, Regan L. 1994. ColE1-compatible vectors for high-level expression of cloned DNAs from the T7 promoter. Gene 144: 59. PDF (scanned)
Munson M, O'Brien R, Sturtevant JM, Regan L. 1994. Redesigning the hydrophobic core of a four-helix-bundle protein. Protein Sci 3: 2015. PDF
Regan L. 1993. What determines where alpha-helices begin and end? Proc Natl Acad Sci USA 90: 10907. PDF
Regan L. 1993. The design of metal-binding sites in proteins. Annu Rev Biophys Biomol Struct 22: 257. PDF (scanned)
Marqusee S, Regan L. 1991. Deconstructing protein structure. Curr Biol 1: 207. PDF (scanned)
Regan L. 1991. Protein design. Curr Opin Biotechnol 2: 544. PubMed
Regan L, Clarke N. 1990. A tetrahedral Zn(II)-binding site introduced into a designed protein. Biochemistry 29: 10878. PDF
Regan L, Ho SP, Wasserman Z, Degrado WF. 1990. Helical proteins. de novo design. Protein Folding: Deciphering the Second Half of the Genetic Code. ed. Gierasch LM, King J. Washington, D.C.: AAAS.
Regan L, DeGrado WF. 1988. Characterization of a helical protein designed from first principles. Science 241: 976. PDF
Regan L, Buxbaum L, Hill K, Schimmel P. 1988. Rationale for engineering an enzyme by introducing a mutation that compensates for a deletion. J Biol Chem 263: 18598. PDF
Frederick CA, Wang AH, Rich A, Regan L, Schimmel P. 1988. Crystallization of a small fragment of an aminoacyl tRNA synthetase. J Mol Biol 203: 521. PubMed
Regan L, Schimmel P. 1987. Selection of aminoacyl tRNA synthetases with enhanced catalytic activity. UCLA Symp Mol Cell Biol 69: 293.
Regan L, Bowie J, Schimmel P. 1987. Polypeptide sequences essential for RNA recognition by an enzyme. Science 235: 1651. PDF
DeGrado WF, Regan L, Ho SP. 1987. The design of a four-helix bundle protein. Cold Spring Harb Symp Quant Biol 52: 521. PubMed
DeGrado WF, Ho S, Wasserman Z, Regan L. 1987. The design and characterization of four-helix-bundle proteins. UCLA Symp Mol Cell Biol 69: 429.
Regan L, Dignam JD, Schimmel P. 1986. A bacterial and silkworm aminoacyl-tRNA synthetase have a common epitope which maps to the catalytic domain of each. J Biol Chem 261: 5241. PDF
Jasin M, Regan L, Schimmel P. 1985. Two mutations in the dispensable part of alanine tRNA synthetase which affect the catalytic activity. J Biol Chem 260: 2226. PDF
Schimmel P, Jasin M, Regan L. 1984. Size polymorphism and the structure of aminoacyl-tRNA synthetases. Fed Proc 43: 2987. PubMed
Jasin M, Regan L, Schimmel P. 1984. Dispensable pieces of an aminoacyl tRNA synthetase which activate the catalytic site. Cell 36: 1089. PubMed
Jasin M, Regan L, Schimmel P. 1983. Modular arrangement of functional domains along the sequence of an aminoacyl tRNA synthetase. Nature 306: 441. PubMed
Questions or Comments? Email Daniel Schlingman or Gili Zilberman