Publications
- Original Research
1.
Pomerat, C.M., Rounds, D.E., Raiborn, C.W.
and Pollard, T.D. (1964) Observations on newborn ratdorsal root ganglia
in
vitro following gamma irradiation. In: "Response of the nervous system
to
ionizingirradiation" (Haley and Snider, eds.), Little, Brown and
Company,
pp. 175-200.
2.
Ito, S., Shihman Chang, R. and Pollard,
T.D. (1969) Cytoplasmic distribution of DNA in a strain of
Hartmannellid
amoeba. J. Protozool. 16:638-645.
3.
Pollard, T.D., Shelton, E., Weihing, R.R. and Korn,
E.D.
(1970) Ultrastructural characterization of F-actin isolated from Acanthamoeba castellanii and
identification of cytoplasmic filaments as F-actin by reaction with
rabbit
muscle heavy meromyosin. J. Mol. Biol. 51:91-97.
4.
Pollard, T.D. and Ito, S. (1970)
Cytoplasmic filaments of Amoeba proteus.
I. The role of filaments in consistency changes and movements. J. Cell
Biol.
46:267-289.
5.
Pollard, T.D. and Weiss, I.W. (1970) Acute
tubular necrosis in a patient with march hemoglobinuria. New Eng.
J. Med.
283:803.
6.
Pollard, T.D. and Korn, E.D. (1971)
Filaments of Amoeba proteus. II.
Binding of heavy meromyosin by thin filaments in motile cytoplasmic
extracts.
J. Cell Biol. 48:216-219.
7.
Stossel, T.P., Pollard, T.D., Mason, R.J.
and Vaughan,
M.
(1971) Isolation and properties of phagocytic vesicles from
polymorphonuclear
leukocytes. J. Clin. Invest. 50:1745-1757.
8.
Adelstein, R.S., Pollard, T.D. and Kuehl,
W.M. (1971) Isolation and characterization of myosin and two myosin
fragments
from human blood platelets. Proc. Natl. Acad. Sci. USA 68:2703-2707.
9.
Stossel, T.P., Mason, R.J., Pollard, T.D.
and Vaughan,
M.
(1972) Isolation and properties of phagocytic vesicles. II. Alveolar
macrophages. J. Clin. Invest. 50:605-614.
10.
Adelstein, R.S., Conti, M.A., Johnson, G.S.,
Pastan, I. and Pollard, T.D. (1972)
Isolation
and characterization of myosin from cloned mouse fibroblasts. Proc.
Natl. Acad.
Sci. USA 69:3693-3697.
11.
Pollard, T.D. and Korn, E.D. (1973) The
contractile proteins of Acanthamoeba
castellanii. Cold
Spring Harbor
Symposium on Quantitative Biology 37:573-583.
12.
Pollard, T.D. and Korn, E.D. (1973) Electron
microscopic identification of actin associated with isolated amoeba
membranes.
J. Biol. Chem. 248:448-450.
13.
Pollard, T.D., Eisenberg, E., Korn, E.D. and
Kielley, W.W. (1973) Inhibition of Mg++‑ATPase of
actin-activated Acanthamoeba myosin by muscle troponin
tropomyosin: implications
for the mechanism of control of amoeba motility and muscle contraction.
Biochem. Biophys. Res. Comm. 51:693-698.
14.
Pollard, T.D. and Korn, E.D. (1973) Acanthamoeba
myosin. I. Isolation from Acanthamoeba castellanii of
an enzyme
similar to muscle myosin. J. Biol. Chem. 248:4682-4690.
15.
Pollard, T.D. and Korn, E.D. (1973) Acanthamoeba
myosin. II. Interaction
with actin and with a new cofactor protein required for actin
activation of Mg++ATPase
activity. J. Biol. Chem. 248:4691-4697.
16.
Stossel, T.P. and Pollard, T.D. (1973)
Myosin in polymorphonuclear leukocytes. J. Biol. Chem. 248:8288-8294.
17.
Orkin, R.W., Pollard, T.D. and Hay, E.D.
(1973) SDS gel analysis of muscle proteins in embryonic cells. Devel.
Biol.
35:388-394.
18.
Burns, R.G. and Pollard, T.D. (1974) A
dynein-like protein from brain. FEBS Letters 40:274-280.
19.
Pollard, T.D., Thomas, S.M. and Niederman,
R. (1974) Human platelet myosin. I. Purification by
a rapid method applicable to other non-muscle
cells, Anal. Biochem. 60:258-266.
20.
Niederman, R. and Pollard, T.D. (1975) Human
platelet myosin. II. In vitro assembly of myosin and structure of
myosin
filaments. J. Cell Biol. 67:72-92.
21.
Pollard, T.D. (1975) Electron microscopy of
synthetic myosin filaments. Evidence for cross-bridge flexibility and
copolymer
formation. J. Cell Biol. 67:93-104.
22.
Woodrum, D.T., Rich, S.A.
and Pollard, T.D. (1975) Evidence for biased bidirectional
polymerization of
actin using heavy meromyosin prepared by an improved method. J. Cell
Biol.
67:231-237.
23.
Pollard, T.D. (1976) The role of actin in
the temperature dependent gelation and contraction of extracts of Acanthamoeba. J. Cell Biol. 68:579-601.
24.
Fujiwara, K. and Pollard, T.D. (1976)
Fluorescent antibody localization of myosin in the cytoplasmic,
cleavage furrow
and mitotic spindle of human cells. J. Cell Biol. 71:848-875.
25.
Pollard, T.D., Fujiwara, K., Handin, R. and
Weiss, G. (1976) Contractile proteins in platelet activation and
contraction.
Annals N.Y. Acad. Sci. 283:218-236.
26.
Schreiner, G.F., Fujiwara, K., Pollard, T.D.
and Unanue, E.R. (1977) Redistribution of myosin accompanying capping
of surface
Ig. J. Exp. Med. 145:1393-1398.
27.
Fujiwara, K. and Pollard, T.D. (1978)
Simultaneous localization of myosin and tubulin in human tissue culture
cells
by double antibody staining. J. Cell Biol. 77:182-195.
28.
Maupin-Szamier, P. and Pollard, T.D. (1978) Actin filament destruction by osmium
tetroxide. J. Cell Biol. 77:837-852.
29.
Herman, I.
and Pollard, T.D. (1978) Actin localization in fixed, dividing cells
stained
with fluorescent heavy meromyosin. Exp. Cell Research 114:15-25.
30.
Pollard, T.D., Stafford,
W.F., III and Porter, M.E. (1978) Characterization of a second myosin
from Acanthamoeba castellanii. J. Biol. Chem.
253:4798-4808.
31.
Griffith,
L.M. and Pollard, T.D. (1978) Evidence for actin filament-microtubule
interaction mediated by microtubule-associated proteins. J. Cell Biol.
78:958-965.
32.
Fujiwara, K., Porter, M.E. and Pollard, T.D.
(1978) Alpha-actinin localization in the cleavage furrow during
cytokinesis. J.
Cell Biol. 79:268-275.
33.
Braun, J., Fujiwara, K., Pollard, T.D. and
Unanue, E.R. (1978) Two distinct mechanisms for redistribution of
lymphocyte
surface macromolecules. I. Relationship to cytoplasmic myosin. J. Cell
Biol.
79:408-418.
34.
Braun, J., Fujiwara, K., Pollard, T.D. and
Unanue, E.R. (1978) Two distinct mechanisms for redistribution of
lymphocyte
surface macromolecules. II. Contrasting effects of local anesthetics
and a
calcium ionophore. J. Cell Biol. 79:419-426.
35.
Mooseker, M.S., Pollard, T.D. and Fujiwara,
K. (1978) Characterization and localization of myosin in the brush
border of
intestinal epithelial cells. J. Cell Biol. 79:444-453.
36.
Herman, I.M. and Pollard, T.D. (1979)
Comparison of purified anti-actin and fluorescent-heavy meromyosin
staining
patterns in dividing cells. J. Cell Biol. 80:509-520.
37.
MacLean-Fletcher, S. and Pollard, T.D.
(1980) Viscometric analysis of the gelation of Acanthamoeba
extracts and purification of two gelation factors. J.
Cell Biol. 85:414-428.
38.
MacLean-Fletcher, S. and Pollard, T.D. (1980)
Mechanism of action of cytochalasin B on actin. Cell 20:329-341.
39.
MacLean-Fletcher, S. and Pollard, T.D.
(1980) Identification of a factor in conventional muscle actin
preparations
which inhibits actin filament self-association. Biochem. Biophys. Res.
Comm.
96:18-27.
40.
Isenberg, G.H., Aebi, U. and Pollard, T.D.
(1980) An actin binding protein from Acanthamoeba
regulates actin filament polymerization and interactions. Nature
288:455-459.
41.
Aebi, U., Smith, P.R., Isenberg, G.H. and
Pollard, T.D. (1980) Structure of crystalline actin sheets. Nature
288:296-298.
42.
Herman, I.M. and Pollard, T.D. (1981)
Electron microscopic localization of cytoplasmic myosin with
ferritin-labeled
antibodies. J. Cell Biol. 88:346-351.
43.
Pollard, T.D. and Mooseker, M.S. (1981)
Direct measurement of actin polymerization rate constants by electron
microscopy of actin filaments nucleated by isolated microvillus cores.
J. Cell
Biol. 88:654-659.
44.
Pollard, T.D. (1981) Purification of a
calcium-sensitive actin gelation protein from Acanthamoeba.
J. Biol. Chem. 256:7666-7670.
45.
Herman, I.M., Crisona, N.J.
and Pollard, T.D. (1981) Relation between cell activity and the
distribution of
cytoplasmic actin and myosin. J. Cell Biol. 90:84-91.
46.
Aebi, U., Fowler, W.E., Isenberg, G.H.,
Pollard, T.D. and Smith, P.R. (1981) Crystalline actin sheets: their
structure
and polymorphism. J. Cell Biol. 91:340-351.
47.
Tseng, P.C.-H. and Pollard, T.D. (1982)
Mechanism of action of Acanthamoeba
profilin. Demonstration of actin species specificity and regulation of
micromolar concentrations of MgCl2. J. Cell Biol.
94:213-218.
48.
Griffith,
L.M. and Pollard, T.D. (1982) Cross-linking of actin filament networks
by
self-association and actin-binding macromolecules. J. Biol. Chem.
257:9135-9142.
49.
Griffith,
L.M. and Pollard, T.D. (1982) The interaction of actin filaments with
microtubules and microtubule-associated proteins. J. Biol. Chem.
257:9143-9151.
50.
Mooseker, M.S., Pollard, T.D. and Wharton,
K.A. (1982) Nucleated polymerization of actin from the
membrane-associated ends
of microvillar filaments in the intestinal brush border. J. Cell Biol.
95:223-233.
51.
Pollard, T.D. (1982) Structure
and polymerization of Acanthamoeba myosin-II
filaments.
J. Cell Biol. 95:816-825.
52.
Maupin, P. and Pollard, T.D. (1983) Improved
preservation and staining of HeLa cell actin filaments, clathrin-coated
membranes, and other cytoplasmic structures by tannic
acid-glutaraldehyde-saponin fixation. J. Cell Biol. 96:51-62.
53.
Wong, A.J., Pollard, T.D. and Herman, I.M.
(1983) Actin filament stress fibers in vascular endothelial cells in vivo. Science 219:867-869.
54.
Dang, C.V., Yang, D.C.H. and Pollard, T.D.
(1983) Association of methionyl-tRNA synthetase with
detergent-insoluble
components of the rough endoplasmic reticulum. J. Cell Biol.
96:1138-1147.
55.
Cooper, J.A., Buhle, E.L., Jr., Walker, S.B., Tsong, T.Y.
and Pollard, T.D. (1983) Kinetic evidence for a monomer activation step
in
actin polymerization. Biochemistry 22:2193-2202.
56.
Cooper, J.A., Walker, S.B. and Pollard, T.D. (1983)
Pyrene
actin: documentation of the validity of a sensitive assay for actin
polymerization. J. Muscle Res. & Cell Motility 4:253-262.
57.
Selden,
S.C. and
Pollard, T.D.
(1983) Phosphorylation of
microtubule-associated proteins regulates their interaction with actin
filaments. J. Biol. Chem. 258:7064-7071.
58.
Smith, P.R., Fowler, W.E., Pollard, T.D. and
Aebi, U. (1983) Structure of the actin molecule determined from
electron
micrographs of crystalline actin sheets with a tentative alignment of
the
molecule in the actin filament. J. Mol. Biol. 167:641-660.
59.
Pollard, T.D. (1983) Measurement of rate
constants for actin filament elongation in solution. Anal. Biochem.
134:406-412.
60.
Tseng, P.C.-H., Runge, M.S., Cooper, J.A.,
Williams, J.C., Jr. and Pollard, T.D. (1984) Physical, immunochemical,
and
functional properties of Acanthamoeba
profilin. J. Cell Biol. 98:214-221.
61.
Pollard, T.D. and Weeds, A.G. (1984) The
rate constant for ATP hydrolysis by polymerized actin. FEBS Lett.
170:94-98.
62.
Kiehart, D.P. and Pollard, T.D. (1984)
Stimulation of Acanthamoeba
actomyosin ATPase activity by myosin-II polymerization. Nature
308:864-866.
63.
Cooper, J.A., Blum, J.D. and Pollard, T.D.
(1984) Acanthamoeba castellanii
capping protein: Properties, mechanism of action, immunologic
cross-reactivity,
and localization. J. Cell Biol. 99:217-225.
64.
Kiehart, D.P., Kaiser, D. and Pollard, T.D.
(1984) Monoclonal antibodies demonstrate limited structural homology
between
myosin isozymes from Acanthamoeba. J.
Cell Biol. 99:1002-1014.
65.
Kiehart, D.P., Kaiser, D. and Pollard, T.D.
(1984) Direct localization of monoclonal antibody-binding sites on Acanthamoeba myosin-II and inhibition of
filament formation by antibodies that bind to specific sites on the
myosin-II
tail. J. Cell Biol. 99:1015-1023.
66.
Kiehart, D.P. and Pollard, T.D. (1984)
Inhibition of Acanthamoeba
actomyosin-II ATPase activity and mechanochemical function by
monoclonal
antibodies. J. Cell Biol. 99:1024-1033.
67.
Pollard, T.D. (1984) Polymerization of
ADP-actin. J. Cell Biol. 99:769-777.
68.
Pollard, T.D. and Cooper, J.A. (1984)
Quantitative analysis of the effect of Acanthamoeba
profilin on actin filament nucleation and elongation. Biochemistry
23:6631-6641.
69.
Pollard, T.D. (1984) Purification of a high
molecular weight actin filament gelation protein from Acanthamoeba
that shares antigenic determinants with vertebrate
spectrins. J. Cell Biol. 99:1970-1980.
70.
Wong, A.J., Kiehart, D.P. and Pollard, T.D.
(1985) Myosin from human erythrocytes. J. Biol. Chem. 260:46-49.
71.
Cooper, J.A. and Pollard, T.D. (1985) Effect
of capping protein on the kinetics of actin polymerization.
Biochemistry:
24:793-799.
72.
Sato, M., Leimbach, G., Schwarz, W.H. and
Pollard, T.D. (1985) Mechanical properties of actin. J. Biol. Chem.
260:8585-8592.
73.
Cooper, J.A., Blum, J.D., Williams, R.C.,
Jr. and Pollard, T.D. (1986) Purification and characterization of
actophorin, a
new 15,000-dalton actin binding protein from Acanthamoeba
castellanii. J. Biol. Chem. 261:477-485.
74.
Kaiser, D.P., Sato, M.D., Ebert, R. and
Pollard, T.D. (1986) Purification and characterization of two isoforms
of Acanthamoeba profilin. J. Cell Biol.
102:221-226.
75.
Selden,
S.C. and
Pollard, T.D. (1986)
Interaction of actin filaments with microtubules is mediated by
microtubule
associated proteins and regulated by phosphorylation. Ann. New York Acad.
Sci. 466:803-812.
76.
Sato, M., Schwarz, W.H. and Pollard, T.D.
(1986) Acanthamoeba profilin affects
the mechanical properties of nonfilamentous actin. J. Biol. Chem.
261:10701-10706.
77.
Adams, R.J.
and Pollard, T.D. (1986) Propulsion of organelles isolated from Acanthamoeba along actin filaments by
myosin-I. Nature 322:754-756.
78.
Drenckhahn, D. and Pollard, T.D. (1986)
Elongation of actin filaments is a diffusion-limited reaction at the
barbed end
and is accelerated by inert macromolecules. J. Biol. Chem.
261:12754-12758.
79.
Magnus, K.A., Lattman, E.E., Sato, M. and
Pollard, T.D. (1986) Crystallization of Acanthamoeba
profilin-I. J. Biol. Chem. 261:13360-13361.
80.
Maupin, P. and Pollard, T.D. (1986)
Arrangement of actin filaments and myosin-like filaments in the
contractile
ring and actin-like filaments in the mitotic spindle of dividing HeLa
cells. J.
Ultrastruct. Res. 94:92-103.
81.
Hagen,
S.J., Kiehart, D.P., Kaiser, D.A. and Pollard, T.D. (1986)
Characterization of
monoclonal antibodies to Acanthamoeba
myosin-I that cross-react with both myosin-II and low molecular weight
nuclear
proteins. J. Cell Biol. 103:2121-2128.
82.
Pollard, T.D. (1986) Rate constants for the
reactions of ATP- and ADP-actin with the ends of actin filaments. J.
Cell Biol.
103:2747-2754.
83.
Pollard, T.D., Tseng, P.C.-H., Rimm, D.L. ,
Bichell, D.P., Williams, R.C., Jr., Sinard, J. and Sato, M. (1986)
Characterization of alpha-actinin from Acanthamoeba.
Cell Motil. Cytoskel. 6:649-661.
84.
Earnshaw, W.C., Sullivan, K.F., Machlin,
P.S., Cooke, C.A., Kaiser, D.A., Pollard, T.D., Rothfield, N.F. and
Cleveland,
D.W. (1987) Molecular cloning of cDNA for CENP‑B, the major human
centromere
autoantigen. J. Cell Biol. 104:817-829.
85.
Sato, M., Schwarz, W.H. and Pollard, T.D.
(1987) Dependence of the mechanical properties of actin/alpha-actinin
gels on
deformation rate. Nature 325:828-830.
86.
Aebi, U. and Pollard, T.D. (1987) A glow
discharge unit to render electron microscope grids and other surfaces
hydrophilic. J. Electron Microscopic Technique 7:29-33.
87.
Sellers, H.S., Schwarz, W.H., Sato, M. and
Pollard, T.D. (1987) Boundary effects on the drag of an oscillating
sphere:
applications to the magnetic sphere rheometer. J. Non-Newtonian Fluid
Mech.
26:43-55.
88.
Ampe, C., Sato, M., Pollard, T.D. and
Vandekerckhove, J. (1988)
The primary structure of the basic isoform of Acanthamoeba
profilin. Eur. J. Biochem. 170:597-601.
89.
Lee, S., Li, M. and Pollard, T.D. (1988) Evaluation
of the
binding of Acanthamoeba profilin to
pyrene-labeled actin by fluorescence enhancement. Anal. Biochem.
168:148-155.
90.
Sato, M., Schwarz, W.H., Selden, S.C.
and Pollard, T.D. (1988)
Mechanical properties of brain tubulin and microtubules. J. Cell Biol.
106:1205-1211.
91.
Murphy, D.B., Gray, R.V., Grasser, W.A. and Pollard,
T.D. (1988)
Direct demonstration of actin filament annealing in vitro.
J. Cell Biol. 106:1947-1954.
92.
Carboni, J.M., Conzelman, K.A., Adams,
R.A., Kaiser, D.A., Pollard, T.D. and Mooseker, M.S. (1988) Structural
and
immunological characterization of the myosin-like 110-kD subunit of the
intestinal microvillar 110K -calmodulin complex: evidence for discrete
myosin
head and calmodulin binding domains. J. Cell Biol. 107:1749-1757.
93.
Rimm, D.L., Pollard, T.D. and Hieter, P. (1988)
Resolution of Acanthamoeba castellanii chromosomes by
pulsed field gel electrophoresis and construction of the initial
linkage map.
Chromosoma 97:219-223.
94.
Magnus, K.A., Maciver, S.K. and Pollard, T.D. (1988)
Crystallization of actophorin, an actin filament severing protein from Acanthamoeba. J. Biol. Chem.
263:18143-18144.
95.
Hitchcock-DeGregori, S.E., Sampath, P. and Pollard,
T.D. (1988)
Tropomyosin inhibits the rate of actin polymerization by stabilizing
actin
filaments. Biochemistry 27:9182-9185.
96.
Sinard, J.H. and Pollard, T.D. (1989) Microinjection
into Acanthamoeba castellanii of monoclonal
antibodies to myosin-II slows but does not stop cell locomotion. Cell
Motil.
Cytoskel. 12:42-52.
97.
Rimm, D.L. and Pollard, T.D. (1989) New plasmid
vectors for high
level synthesis of eukaryotic fusion proteins in Escherichia coli. Gene
75:323-327.
98.
Rimm, D.L., Sinard, J.H. and Pollard, T.D. (1989)
Location of the
head-tail junction of myosin. J. Cell Biol. 108:1783-1789.
99.
Vandekerckhove, J.S., Kaiser, D.A. and Pollard, T.D.
(1989) Acanthamoeba actin and profilin can be
crosslinked between glutamic acid 364 of actin and lysine 115 of
profilin.
J. Cell Biol. 109:619-626.
100.
Adams, R.J. and
Pollard, T.D
(1989) Binding of myosin I to membrane
lipids. Nature 340:565-568.
101.
Rimm, D.L. and Pollard, T.D.. (1989) Purification
and
characterization of an Acanthamoeba
nuclear actin-binding protein. J. Cell Biol. 109:585-591.
102.
Sinard, J.H. and Pollard, T.D. (1989) The effect of
heavy chain
phosphorylation and solution conditions on the assembly of Acanthamoeba
myosin-II. J. Cell Biol. 109:1529-1535.
103.
Sinard, J.H., Stafford,
W.F. and
Pollard, T.D. (1989) The mechanism of assembly of Acanthamoeba
myosin-II minifilaments: minifilaments assemble by
three successive dimerization steps. J. Cell Biol. 109:1537-1547.
104.
Kaiser, D.A., Goldschmidt-Clermont, P.J., Levine, B.
and Pollard,
T.D. (1989) Characterization of renatured profilin purified by urea
elution
from poly-L-proline agarose columns. Cell Motil. Cytoskel. 14:251-262.
105.
Sinard, J.H. and Pollard, T.D. (1990)
Acanthamoeba
myosin-II minifilaments assemble on a millisecond time scale with rate
constants greater than those expected for a diffusion limited reaction.
J.
Biol. Chem. 265:3654-3660.
106.
Goldschmidt-Clermont, P.J., Machesky, L.M.,
Baldassare, J.J. and
Pollard, T.D. (1990) The actin-binding
protein profilin binds to PIP2 and
inhibits its hydrolysis by phospholipase C. Science 247:1575-1578.
107.
Pollard, T.D., Maupin, P., Sinard, J. and Huxley,
H.E. (1990) A
stopped-flow/rapid-freezing machine with millisecond time resolution to
prepare
intermediates in biochemical reactions for electron microscopy. J.
Electron
Microscopic Technique 16:160-166.
108.
Rimm, D.L., Kaiser, D.A., Bhandari, D., Maupin, P.,
Kiehart, D.P.
and Pollard, T.D. (1990) Identification
of functional regions on the tail of Acanthamoeba
myosin-II using recombinant fusion proteins. I. High resolution epitope
mapping
and characterization of monoclonal antibody binding sites. J. Cell
Biol.
111:2405-2416.
109.
Sinard, J.H., Rimm, D.L. and Pollard, T.D. (1990) Identification of functional regions on the
tail of Acanthamoeba myosin-II using
recombinant fusion proteins. II. Assembly properties of tails with NH2- and
COOH-terminal deletions. J. Cell Biol. 111:2417-2426.
110.
Machesky, L.M., Goldschmidt-Clermont, P.J. and
Pollard, T.D. (1990)
The affinities of human platelet and Acanthamoeba
profilin isoforms for polyphosphoinositides account for their relative
abilities to inhibit phospholipase C. Cell Regulation 1:937-950.
111.
Sampath, P. and Pollard, T.D. (1991)
Effects of cytochalasin, phalloidin and pH on
the elongation of actin filaments. Biochemistry 30:1973-1980.
112.
Goldschmidt-Clermont, P.J., Kim, J.W., Machesky,
L.M., Rhee, S.G.
and Pollard, T.D. (1991) Regulation of
phospholipase C-gamma l by profilin and tyrosine phosphorylation.
Science
251:1231-1233.
113.
Goldschmidt-Clermont, P.J., Machesky, L.M.,
Doberstein, S.K. and
Pollard, T.D. (1991) Mechanism of the
interaction of human platelet profilin with actin. J. Cell Biol.
113:1081-1089.
114.
Vojtek, A., Haarer, B., Field, J., Gerst, J.,
Pollard, T.D., Brown,
S. and Wigler, M. (1991) Evidence for a
functional link between profilin and CAP in the yeast Saccharomyces
cerevisiae. Cell 66:497-505.
115.
Pollard, T.D. and Rimm, D.L. (1991)
Analysis of cDNA clones for Acanthamoeba
profilin-I and profilin-II shows end to end homology with vertebrate
profilins
and a family of profilin genes. Cell Motil. Cytoskel. 20:169-177.
116.
Bremer, A., Millonig, R.C., Sutterlin, R., Engel,
A., Pollard, T.D.
and Aebi, U. (1991) The structural basis
for the intrinsic disorder of the actin filament: the 'lateral
slipping' model.
J. Cell Biol. 115:689-703.
117.
Kobayashi, T., Zot, H.G., Pollard, T.D. and Collins,
J.H.
(1991) Functional implications of the
unusual amino acid sequence of the regulatory light chain of Acanthamoeba castellanii myosin‑II. J.
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118.
Maciver, S.K., Zot, H.G. and Pollard, T.D. (1991) Characterization of actin filament severing
by actophorin from Acanthamoeba
castellanii. J. Cell Biol. 115:1611-1620.
119.
Maciver, S.K., Wachsstock, D., Schwarz, W.H. and
Pollard, T.D.
(1991) The actin filament severing
protein actophorin catalyzes the formation of rigid bundles of actin
filaments
crosslinked with alpha-actinin. J. Cell Biol. 115:1621-1628.
120.
Zot, H.G., Doberstein, S.K. and Pollard, T.D. (1991) Myosin-I moves actin filaments on a
phospholipid substrate: implications for membrane targeting. J. Cell
Biol.
116:367-376.
121.
Doberstein, S.K. and Pollard, T.D. (1992) Localization and specificity of the
phospholipid and actin binding sites on the tail of Acanthamoeba
myosin-IC. J. Cell Biol. 117:1241-1249.
122.
Yonemura, S. and Pollard, T.D. (1992)
The localization of actin, myosin-I and
myosin-II in Acanthamoeba by
fluorescence microscopy. J. Cell Sci. 102:629-642.
123.
Satterwhite, L.L., Lohka, M.L., Wilson, K., Cisek, L.J., Corden, J.L.
and
Pollard, T.D. (1992) Phosphorylation of
myosin regulatory light chain by cyclin-p34 kinase. J. Cell Biol.
118:595-605.
124.
Goldschmidt-Clermont, P.J., Furman, M.I.,
Wachsstock, D., Safer,
D., Nachmias, V.T. and Pollard, T.D. (1992) Regulation
of actin nucleotide exchange by
thymosinß4 and profilin.
Molec. Biol. Cell 3:1015-1024.
125.
Pollard, T.D., Goldberg, I.
and
Schwarz, W.H. (1992) Nucleotide
exchange, structure and mechanical properties of filaments assembled
from
ATP-actin and ADP-actin. J. Biol. Chem. 267:20339-20345.
126.
Pollard, T.D., Bhandari, D., Maupin, P., Weeds, A.G.
and Zot, H.G.
(1993) Direct visualization by electron
microscopy of the weakly-bound intermediates in the actomyosin ATPase
cycle.
Biophys. J. 64:454-471.
127.
Archer, S.J., Vinson, V.K., Pollard, T.D. and
Torchia, D.A.
(1993) Secondary structure and topology
of Acanthamoeba profilin-I
as determined by heteronuclear
magnetic resonance spectroscopy. Biochemistry. 32:6680-6687.
128.
Wachsstock, D.H., Schwarz, W.H. and Pollard, T.D.
(1993) Affinity of alpha-actinin for actin
filaments
determines the structure and mechanical properties of actin filament
gels.
Biophys. J. 65:205-214.
129.
Quirk, S., Maciver, S.K., Ampe, C., Doberstein,
S.K., Kaiser, D.A.,
VanDamme, J., Vandekerckhove, J.S. and Pollard, T.D. (1993) Primary structure and studies of Acanthamoeba
actophorin. Biochemistry
32:8525-8533.
130.
Vinson, V.K., Archer, S.J., Lattman, E.E., Pollard,
T.D. and
Torchia, D.A. (1993) Three dimensional
solution structure of Acanthamoeba
profilin-I J. Cell
Biol. 122:1277-1283.
131.
Doberstein, S.K., Baines, I., Weigand, G., Korn,
E.D. and Pollard,
T.D. (1993) Inhibition of contractile
vacuole function in vivo by
myosin-I antibodies. Nature 365: 841-843.
132.
Porter, J., Yu, M., Doberstein, S.K., Pollard, T.D.
and Montell, C.
(1993) Dependence of calmodulin
localization in the retina on the NINAC unconventional myosin. Science 262:1038-1042.
133.
Archer, S.J., Vinson, V.K., Pollard, T.D. and
Torchia, D.A. (1994) Elucidation of the
poly-L-proline binding
site in Acanthamoeba profilin-I by
NMR spectroscopy. FEBS Lett. 337:145-151.
134.
Almo,
S.C., Pollard,
T.D., Way, M. and Lattman,
E.E. (1994) Purification,
characterization and crystallization of Acanthamoeba profilin
expressed in Escherichia
coli. J. Mol. Biol. 236:950-952.
135.
Wachsstock, D.H., Schwarz, W.H. and Pollard, T.D.
(1994) Crosslinker dynamics determine the
mechanical
properties of actin gels. Biophys. J. 66:801-809.
136.
Smith, K.J., Levy, D.B., Maupin, P., Pollard, T.D.,
Vogelstein, B.
and Kinzler, K.W. (1994) Wild-type but
not mutant APC associates with the microtubule cytoskeleton. Cancer
Res.
54:3672-3675.
137.
Wachsstock, D.H. and Pollard, T.D. (1994) Transient state kinetics tutorial using
KINSIM.
Biophys. J. 67:1260-1273.
138.
Fedorov, A.A., Magnus, K.A., Graupe, H., Lattman,
E.E., Pollard,
T.D., and Almo, S.C.
(1994) Crystal structures of two Acanthamoeba
profilins that differ in
their affinity for polyphosphoinosides. Proc. Nat. Acad. Sci. USA.
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139.
Machesky, L.M., Cole, N.B., Moss, B. and Pollard,
T.D. (1994) Vaccinia virus expresses a
novel profilin
with a higher affinity for polyphosphoinositides than for actin.
Biochemistry
33:10815-10824.
140.
Machesky, L.M., Atkinson, S.J., Ampe, C.,
Vandekerckhove, J. and
Pollard, T.D. (1994) A cortical complex
of seven Acanthamoeba polypeptides
including two unconventional
actins binds to profilin. J. Cell Biol. 127:107-115.
141.
Maupin, P., Phillips, C.L., Adelstein, R.S. and
Pollard, T.D.
(1994) Differential localization of
myosin‑II isozymes in human cultured cells and blood cells. J. Cell
Sci.
107:3077-3090.
142.
De La Cruz, E. and Pollard, T.D. (1994)
Transient kinetic analysis of rhodamine
phalloidin binding to actin filaments. Biochemistry 33:14387-14392.
143.
De La Cruz, E.M. and Pollard, T.D. (1995) Nucleotide and divalent cation-free
actin: Stabilization by sucrose and
nucleotide binding kinetics. Biochemistry 34:5452-5461.
144.
Doberstein, S.K., Wiegand, G., Machesky, L.M. and
Pollard, T.D.
(1995) Fluorescent erythrocyte ghosts as
standards for quantitative flow cytometry. Cytometry 20:14-18.
145.
Kelleher, J.F., Atkinson, S.J. and Pollard, T.D.
(1995) Sequences, structural models and
cellular
localization of the actin-related proteins Arp2 and Arp3 from Acanthamoeba.. J. Cell Biol.
131:385-397.
146.
Bresnick, A., Wolff-Long, V., Baumann, O. and
Pollard, T.D.
(1995) Phosphorylation on threonine-18
of the regulatory light chain dissociates the ATPase and motor
properties of
smooth muscle myosin-II. Biochemistry 34:12576-12583.
147.
Kaiser, D.A. and Pollard, T.D. (1996)
Characterization of the actin and
poly-L-proline binding sites of Acanthamoeba
profilin with monoclonal antibodies and mutagenesis. J. Mol. Biol.
255:89-107.
148.
Ostap, E. M. and Pollard, T.D. (1996)
Biochemical kinetic characterization of the Acanthamoeba myosin‑I ATPase. J. Cell
Biol. 132:1053-1060.
149.
De La Cruz, E.M. and Pollard, T.D. (1996) Kinetics and thermodynamics of phalloidin
binding to actin from three divergent species. Biochemistry
35:14054-14061.
150.
Petrella, E.C., Machesky, L.M., Kaiser, D.A. and
Pollard, T.D.
(1996) Structural requirements and
thermodynamics of the interaction of proline peptides with profilin.
Biochemistry 35:16535-16543.
151.
Mullins, R.D., Stafford,
W.F. and
Pollard, T.D. (1997) Structure, subunit
topology and actin-binding activity of the Arp2/3 complex from Acanthamoeba. J. Cell Biol. 136:
331-343.
152.
Turbedsky, K., Pollard, T.D. and Bresnick, A.R.
(1997) A subset of protein kinase C
phosphorylation
sites on myosin II regulatory light chain inhibit phosphorylation by
myosin
light chain kinase. Biochemistry 36: 2063-2067.
153.
Leonard, S.A.,
Gittis, A.G., Petrella, E.C., Pollard, T.D. and Lattman, E.E. (1997) Crystal
structure of the actin-binding protein actophorin from Acanthamoeba.
Nature Struct. Biol. 4:369-373.
154.
Bezanilla, M., Forsburg, S.L. and Pollard, T.D.
(1997) Identification of a second
myosin-II in S.
pombe: Myp2p is conditionally required
for cytokinesis. Molec. Biol. Cell 8:2693-2705.
155.
Jontes, J.D., Milligan, R.A., Pollard, T.D. and
Ostap, E.M. (1997)
Kinetic characterization of brush border myosin-I ATPase. Proc. Nat.
Acad. Sci.
USA 94:14332-14337.
156.
Xu, J., Wirtz, D. and Pollard, T.D. (1998) Dynamic crosslinking by alpha-actinin
determines the mechanical properties of actin filament networks. J.
Biol. Chem.
273:9570-9576.
157.
Xu, J., Schwarz, W.H., Kas, J.A., Stossel, T.A.,
Janmey, P.A. and
Pollard, T.D. (1998) Mechanical properties
of actin filament networks depend on preparation, polymerization
conditions and
storage of G-actin. Biophys. J. 74:2731-2740.
158.
Mullins, R.D., Kelleher, J.F., Xu, J. and Pollard,
T.D. (1998)
Arp2/3 complex from Acanthamoeba
binds profilin and crosslinks actin filaments. Molec. Biol. Cell
9:841-852.
159.
Jontes, J.D., Ostap, E.M., Pollard, T.D. and
Milligan, R.A. (1998)
Three-dimensional structure of Acanthamoeba
myosin-IB determined by cryo-electron microscopy of decorated actin
filaments.
J. Cell Biol. 141:155-162.
160.
Mullins, R.D., Heuser, J.A. and Pollard, T.D. (1998) The interaction of the Arp2/3 complex with
actin: nucleation, high affinity pointed
end capping and formation of branching networks of filaments. Proc.
Nat. Acad.
Sci. USA 95:6181-6186.
161.
Vinson*, V.K., De La Cruz*, E.M., Higgs, H. and
Pollard, T.D.
(1998) Interactions of Acanthamoeba
profilin with actin and
nucleotides bound to actin. Biochemistry 37:10871-10880.* co-first
authors.
162.
Freeman, J.L.R., De La Cruz, E.M., Pollard, T.D.,
Lefkowitz, R.J.
and Pitcher, J.A. (1998) Regulation of
G-protein coupled receptor kinase 5 (GRK5) by actin. J. Biol. Chem.
273:20653-20657.
163.
Blanchoin, L. and Pollard, T.D. (1998)
Interaction of actin monomers with actophorin
(ADF/cofilin) and profilin. J. Biol. Chem. 273:25106-25111.
164.
Liu, S., Fedorov, A.A., Pollard, T.D., Lattman,
E.E., Almo, S.C.
and Magnus, K.A. (1998) Crystal packing
induces a conformational change in profilin-I from Acanthamoeba
castellanii. J. Struct. Biol. 123:22-29.
165.
Xu, J., Casella, J.F. and Pollard, T.D. (1999) Effect of capping protein, CapZ, on the
length of actin filaments and mechanical properties of actin filament
networks.
J. Cell Motility Cytoskeleton 42:73-81.
166.
Machesky, L.M., Mullins, R.D., Higgs, H.N., Kaiser,
D.A.,
Blanchoin, L., May, R.C., Hall, M.E. and Pollard, T.D. (1999) WASp-related
protein Scar activates
dendritic nucleation of actin filaments by Arp2/3 complex. Proc. Nat.
Acad Sci.
USA 96: 3739-3744.
167.
Mullins, R.D. and Pollard, T.D. (1999)
Rho-family GTPases require Arp2/3 complex to
stimulate actin polymerization in Acanthamoeba
extracts. Current Biol. 9:405-415.
168.
Blanchoin, L. and Pollard, T.D. (1999)
Mechanism of interaction of Acanthamoeba
actophorin (ADF/cofilin)
with actin filaments. J. Biol. Chem. 274:15538-15546.
169.
May, R.C., Hall, M.E., Higgs, H.N., Pollard, T.D.,
Chakraborty, T.,
Wehland, J., Machesky, L.M. and Sechi, A.S. (1999)
The Arp2/3 complex is essential for the
actin-based motility of Listeria
monocytogenes. Current Biol. 9:759-762.
170.
Kaiser, D.A., Vinson, V.K., Murphy, D.B. and
Pollard, T.D. (1999)
Profilin is predominantly associated with monomeric actin in Acanthamoeba.. J. Cell Science
112:3769-3777.
171. Higgs,
H.N., Blanchoin, L. and Pollard, T.D. (1999) Influence of the
Wiskott-Aldrich syndrome protein (WASp) C terminus and Arp2/3 complex
on actin
polymerization. Biochemistry 38:15212-15222.
172.
Lee, W.-L., Ostap, E.M., Zot, H.G. and Pollard, T.D.
(1999)
Organization and ligand binding properties of the tail of Acanthamoeba
myosin-IA: Identification of an actin
binding site in the basic
(TH-1) domain. J.
Biol. Chem. 274: 35159-15171.
173.
Sept, D., Xu, J., Pollard, T.D. and McCammon, J.A.
(1999) Annealing
accounts for the length of actin filaments formed by spontaneous
polymerization. Biophys. J. 77:2911-2919.
174.
Blanchoin, L., Robinson, R.C., Choe, S. and Pollard,
T.D. (2000)
Phosphorylation of Acanthamoeba
actophorin (ADF/cofilin) blocks interaction with actin without a change
in
atomic structure. J. Molec. Biol. 295:203-211.
175.
De La Cruz, E.M., Mandinova, A., Steinmetz, M.O.,
Stoffler, D.,
Aebi, U. and Pollard, T.D. (2000) Polymerization and structure of
nucleotide-free actin filaments. J. Molec. Biol. 295:517-526.
176.
Bezanilla, M. and Pollard, T.D. (2000) Myosin-II
tails confer
unique functions in S. pombe: characterization
of a novel myosin-II tail.
Molec. Biol. Cell 11:79-91.
177.
Bezanilla, M., Wilson,
J.M. and Pollard, T.D. (2000) Fission
yeast myosin-II isoforms assemble into contractile rings at distinct
times
during mitosis. Current Biology 10:397-400.
178.
Blanchoin*, L., Amann*, K. J., Higgs, H.N.,
Marchand, J.-B.,
Kaiser, D.A. and Pollard, T.D. (2000) Direct observation of dendritic
actin
filament networks nucleated by Arp2/3 complex and WASp/Scar proteins.
Nature
404:1007-1011. * co-first authors.
179.
Higgs, H. N. and Pollard, T. D. (2000) Activation by
Cdc42 and PIP2 of Wiskott-Aldrich Syndrome protein (WASp)
stimulates actin nucleation by Arp2/3
complex. J. Cell Biol. 150: 1311-1320.
180. Blanchoin,
L., Pollard, T.D. and Mullins, R.D. (2000) Interactions
of actophorin (ADF/cofilin), Arp2/3 complex, capping protein and
profilin in
the remodeling and disassembly of branched actin filament networks.
Current
Biology. 10:1273-1282.
181.
Kim, Y.J., Kaiser, D.A., Pollard, T.D. and Ichikawa, Y.
(2000) Synthesis of (3R)-carboxy
pyrrolidine (a ß-proline analog) and its oligomer. Bioorg. Med. Chem.
Lett.
10:2417-2419.
182.
Lee, W.-L., Bezanilla, M. and Pollard, T.D. (2000)
Fission yeast
myosin-I, Myo1p, stimulates actin assembly by Arp2/3 complex and shares
functions with WASp. J. Cell Biol. 151:789-800.
183.
Marchand, J.-B., Kaiser, D. A., Pollard, T. D. and
Higgs, H. N.
(2001) Interaction of WASp/Scar proteins with actin and vertebrate
Arp2/3
complex. Nature Cell Biol. 3:76-82.
184.
Amann, K.J. and Pollard, T.D. (2001) The Arp2/3
complex nucleates
filament branches from the sides of pre-existing actin filaments.
Nature Cell
Biol. 3:306-310.
185.
Pollard, T.D. (2001) Genomics, the cytoskeleton and
motility.
Nature 409:842-843.
186.
Lu, J. and Pollard, T.D. (2001) Profilin binding to
poly-L-proline
and actin monomers along with ability to catalyze actin nucleotide
exchange are
required for viability of fission yeast. Molec. Biol. Cell.
12:1161-1175.
187.
Heufner, K., Higgs, H.N., Pollard, T.D., Jacobi, C.,
Aepfelbacher,
M. and Linder, S. (2001) The VC region of Wiskott-Aldrich syndrome
protein
induces Arp2/3 complex-dependent actin nucleation. J. Biol. Chem.
276:35761-35767.
188.
Volkmann, N., Amann, K.J., Stoilova-McPhie, S.,
Egile, C., Winter,
D.C., Hazelwood, L., Heuser, J.E., Li, R., Pollard, T.D. and Hanein, D.
(2001)
Structure of Arp2/3 complex in its activated state and in actin
filament branch
junctions. Science 293:2456-2459.
189.
Blanchoin. L., Pollard, T.D. and
Hitchcock-DeGregori, S.E. (2001)
Inhibition of Arp2/3
Complex-Nucleated Actin Polymerization and Branch Formation by
Tropomyosin.
Current Biol. 11:1300-1304.
190.
Andrianantoandro, E., Blanchoin, L., Sept, D.,
McCammon, J.A. and
Pollard, T.D. (2001) Kinetic mechanism of end to end annealing of actin
filaments. J. Molec. Biol. 312:721-730.
191.
Robinson*, R.C., Turbedsky*, K., Kaiser, D.A.,
Higgs, H.N.,
Marchand, J.-B., Choe, S. and Pollard, T.D. (2001) Crystal structure of Arp2/3 complex.
Science
294:1679-1684. * co-first authors.
192.
Amann, K.J. and Pollard, T.D. (2001) Direct
real-time observation
of actin filament branching mediated by Arp2/3 complex using total
internal
reflection microscopy. Proc. Nat. Acad. Sci. (U.S.A.)
98:15009-15013.
193.
Blanchoin, L. and Pollard, T.D. (2002) Hydrolysis of
bound ATP by
polymerized actin depends on the bound divalent cation but not
profilin.
Biochemistry 41:597-602.
194.
Okada, K., Blanchoin, L., Abe, H., Chen, H.,
Pollard, T.D. and Bamburg,
J.R. (2002) Xenopus actin interacting protein 1 (XAip1) enhances
cofilin
fragmentation of filaments by capping filament ends. J. Biol. Chem.
277:
43011-43016
195.
Kong, H.-H. and Pollard, T.D. (2002) Intracellular
localization of
myosin-II and myosin-IC in live Acanthamoeba
by transient transfection of EGFP fusion proteins. J. Cell Sci.
115:4993-5002.
196.
Jahng, A.W., Strang, C., Kaiser, D.A., Pollard,
T.D., Pfaffinger,
P. and Choe, S. (2003) Zinc mediates assembly of the T1 domain of the
voltage-gated
K channel 4.2. J. Biol. Chem. 278: 47885- 47890.
197.
Ostap, E.M., Maupin, P., Doberstein, S.K., Baines,
I.C., Korn, E.D.
and Pollard, T.D. (2003) Dynamic localization of myosin-I to endocytic
structures in Acanthamoeba. Cell
Motil. Cytoskel. 54:29-40.
198.
Maul,R.S., Song, Y., Amann, K.J., Gerbin, S.C.,
Pollard, T.D. and Chang, D.D. (2003) EPLIN regulates actin dynamics by
crosslinking and stabilizing filaments. J. Cell Biol. 160:399-407.
199.
Kovar, D.R., Kuhn, J.R., Tichy, A. and Pollard, T.D.
(2003) The
fission yeast cytokinesis formin Cdc12p is a barbed end actin filament
capping
protein gated by profilin. J. Cell Biol. 161:875-887.
200.
Panchal,
S.C., Kaiser,
D.A., Torres, E., Pollard,
T.D. and Rosen, M.K. (2003) A conserved amphipathic helix in WASP/Scar
proteins
is essential for activation of Arp2/3 complex. Nature Struct. Biol.
10:591-598.
201.
Wu, J.-Q., Kuhn, J.R., Kovar, D.R. and Pollard, T.D.
(2003) Spatial
and temporal pathway for assembly and constriction of the contractile
ring in
fission yeast cytokinesis. Devel. Cell 5:723-734.
202.
Beltzner, C.C. and Pollard, T.D. (2004)
Identification of
functionally important residues of Arp2/3 complex by analysis of
homology
models from diverse species. J. Molec. Biol. 336:551-565.
203.
Golemi-Kotra, D., Mahaffy, R., Footer, M.J.,
Holtzman, J.H.,
Pollard, T.D., Theriot, J.A. and Schepartz, A. (2004) High affinity,
paralog-specific recognition of the Mena EVH1 domain by a miniature
protein. J.
Amer. Chem. Soc. 126:4-5.
204,
Kovar, D.R. and Pollard, T.D. (2004) Insertional
assembly of actin
in association with formins produces piconewton forces. Proc. Nat.
Acad. Sci.
USA 101:14725-14730.
205.
Lord, M.
and Pollard, T.D. (2004) UCS protein Rng3 activates actin filament
gliding by
fission yeast myosin-II. J. Cell Biol. 167:315-325.
206.
Nolen, B.,
Littlefield, R.S. and Pollard, T.D. (2004) Crystal structures of bovine Arp2/3
complex
with bound ATP or ADP. Proc. Nat. Acad. Sci. USA 101:15627-15632.
207.
Turbedsky,
K. and Pollard, T.D. (2005) Assembly of Acanthamoeba
myosin-II minifilaments. I. Definition of C-terminal residues required
to form
coiled-coils, dimers and octamers J. Molec. Biol. 345:351-361.
208.
Turbedsky,
K., Pollard, T.D. and Yeager, M. (2005) Assembly of Acanthamoeba
Myosin-II minifilaments. II. Model of anti-parallel
dimers based on EM and X-ray diffraction of 2D and 3D crystals. J. Mol.
Biol.
345:363-373.
209.
Kuhn, J.R. and Pollard, T.D. (2005) Analysis of
actin filament
dynamics by total internal reflection fluorescence microscopy.
Biophysical J.
88:1387-1402.
210.
Kovar, D.R., Wu, J.-Q. and Pollard, T.D. (2005)
Profilin-mediated
competition between capping protein and formin Cdc12p during
cytokinesis in
fission yeast. Molec. Biol. Cell, 16:2313-2324.
211.
Sirotkin, V., Beltzner, C.C., Marchand, J.B. and
Pollard, T.D.
(2005) Interactions of WASp, myosin-I, and
verprolin
with Arp2/3 complex during actin patch assembly in fission yeast.
J.
Cell Biol. 170:637-648.
212.
Wu, J.-Q. and Pollard, T.D. (2005) Counting
cytokinesis proteins
globally and locally in fission yeast. Science 310:310-314.
213.
Lord, M., Laves, E. and Pollard, T.D. (2005)
Cytokinesis depends on
the motor domains of myosin-II in fission yeast but not in budding
yeast.
Molec. Biol. Cell 16:5346-5355.
214.
Kovar, D.R., Harris, E.S., Mahaffy, R.E. Higgs, H.N.
and Pollard,
T.D. (2006) Control of the assembly of ATP- and ADP-actin by formins
and
profilin. Cell 724:423-435.
215.
Vavylonis, D., Kovar, D.R., O'Shaughnessy, B. and
Pollard, T.D.
(2006) Mathematical model of the assembly of ATP- and ADP-actin by
formins and
profilin. Molec. Cell 21:455-466.
216.
Haviv, L., Brill-Karniely, Y., Mahaffy, R.,
Backouche, F.,
Ben-Shaul, A., Pollard, T.D. and
Bernheim-Groswasser, A. (2006) Reconstitution of the transition from
lamellipodium to filopodium in a membrane free system. Proc. Nat. Acad.
Sci.
USA 103:4906-4911.
217.
Moseley, J.B., Okada, K., Balcer, H.I., Kovar, D.R.,
Pollard, T.D.
and Goode, B.L. (2006) Twinfilin is an actin filament severing protein
and
promotes rapid turnover of actin structures in vivo. J. Cell Sci.
119:1547-1557.
218.
Wu, J.-Q., Sirotkin, V., Kovar, D., Lord, M.,
Beltzner, C., Kuhn,
J.R. and Pollard, T.D. (2006) Assembly
of the cytokinetic contractile ring from a broad band of nodes in
fission
yeast. J. Cell Biol. 174:391-402.
219.
Andrianantoandro, E. and Pollard, T.D. (2006)
Mechanism of actin
filament turnover by severing and nucleation at different
concentrations of
ADF/cofilins. Molec. Cell 24:13-23.
220.
Mahaffy, R.E. and Pollard, T.D. (2006) Kinetics of
the formation
and dissociation of actin filament branches by
Arp2/3 complex. Biophys. J. 91, 3519-28.
221.
Kiselar, J.G, Mahaffy, R., Pollard, T.D., Almo, S.C.
and Chance, M.R. (2007) Local and global conformational rearrangements
of
Arp2/3 complex mediated by binding of nucleotides and WASp. Proc. Nat.
Acad.
Sci. USA. In press.
222.
Ali, M.Y., Krementsova, E.B., Kennedy, G.G.,
Mahaffy, R., Pollard,
T.D., Trybus, K.M. and Warshaw, D.M. (2007) Myosin V maneuvers through
actin
intersections and diffuses along microtubules. Proc. Nat. Acad. Sci.
USA. In
press.
Publications
- Reviews and Invited Chapters
1.
Pollard, T.D. (1972) Motile cytoplasmic extracts of Amoeba proteus. Acta Protozool.
11:55-58.
2.
Pollard, T.D., Adelstein, R.S. and Korn, E.D. (1972) Isolation of myosin from Acanthamoeba
castellanii and human platelets. Acta Protozool.
11:59-66.
3.
Pollard, T.D. (1973) Progress in understanding
amoeboid
movement at the molecular level. In: "The Biology of the Amoeba" (K.
Jeon, ed.), Academic Press, Inc., New York, pp. 291-317.
4.
Pollard, T.D. and Weihing, R.R. (1974) Actin and
myosin and
cell movement. CRC Critical Reviews in Biochemistry 2:1-65.
5.
Pollard, T.D. (1975) Functional implications of the
biochemical
and structural properties of cytoplasmic contractile proteins. In:
"Molecules and Cell Movement" (S. Inoue and R.E. Stephens, eds.),
Raven Press, New York,
pp. 259-269.
6.
Pollard, T.D. (1976) The properties of actin, myosin
and
associated proteins from nonmuscle cells. In: "Handbook of Biochemistry
and Molecular Biology", Proteins II, (G. Fasman, ed.), CRC Press, Cleveland, Ohio;
pp. 307-324.
7.
Pollard, T.D., Fujiwara, K., Niederman, R. and
Maupin-Szamier,
P. (1976) Evidence for the role of cytoplasmic actin and myosin in
cellular
structure and motility. In: "Cell Motility" (R. Goldman,
T.D. Pollard and J. Rosenbaum, eds.), Cold Spring
Harbor Laboratory
Press, New York,
pp. 689-724.
8.
Pollard, T.D. and Fujiwara, K. (1976) Participation
of
contractile proteins in cytoplasmic structure and cell division.
Current Topics
in Intracellular Regulation. In: "Contractile Systems in Non-muscle
Tissues", (S.V. Perry, A. Margreth and R.S. Adelstein, eds.),
Elsevier/North-Holland Biomedical Press, Amsterdam,
pp. 23-28.
9.
Pollard, T.D. (1976) Cytoskeletal functions of
cytoplasmic
contractile proteins. J. Supramolec. Str. 5:317-334.
10.
Pollard, T.D. (1977) Cytoplasmic contractile
proteins. In:
"International Cell Biology 1976-1977" (B.R. Brinkley and K.R.
Porter, eds.), Rockefeller University Press, New York, pp. 378-387.
11.
Pollard, T.D. and Maupin, P. (1978) Electron
microscopy of
cytoplasmic contractile proteins. In: "Electron Microscopy", Vol. 3,
(G.W. Bailey, ed.), Claitor's Publ. Co. Baton, Rouge, LA, pp. 606-614.
12.
Adelstein, R.S. and Pollard, T.D. (1978) Platelet
contractile
proteins. In: "Progress in Hemostasis and Thrombosis", (T.H. Spaet,
ed.), Grune & Stratton Press, New York, pp. 37-58.
13.
Pollard, T.D. (1978) Contractile proteins and cell
surface
dynamics. In: "Transport of Macromolecules in Cellular Systems",
(S.C. Silverstein, ed.), Dahlem Konferenzen Publications, Berlin, pp.
489-502.
14.
Pollard, T.D. (1979) Cytoplasmic myosin filaments.
In:
"Motility in Cell Function", (F.A. Pepe, J.W. Sanger and V.T.
Nachmias, eds.), Academic Press, Inc., New York, pp. 117-125.
15.
Pollard, T.D. (1980) Platelet contractile proteins.
In:
"Thrombosis and Haemostasis" 42:1634-1637.
16.
Fujiwara, K. and Pollard, T.D. (1980) Techniques for
localizing
contractile proteins with fluorescent antibodies. In: "Current Topics
in
Developmental Biology", Vol. 14, Academic Press, Inc., New York, pp.
271-296.
17.
Pollard, T.D. (1981) Cytoplasmic contractile
proteins. In:
"Discovery in Cell Biology", J. Cell Biol. 91:156s-165s.
18.
Paranko, J., Pelliniemi, L.J., Dym, M., Fujiwara, K.
and Pollard,
T.D. (1981) Postnatal development of myosin containing cells in the
male rat
reproductive tract. In: "International Congress Series No. 559,
Development and Function of Reproductive Organs", Proceedings of the
Vth
Workshop on the Development and Function of the Reproductive Organs, Copenhagen, (A.G. Byskov and H. Peters, eds.),
Excerpta
Medica, Elsevier North-Holland, Amsterdam,
pp. 191-198.
19.
Pollard, T.D. and Craig, S.W. (1982) Mechanism of
actin
polymerization. Trends in Biochem. Sci. 7:55-58.
20.
Craig, S.W. and Pollard, T.D. (1982) Actin-binding
proteins.
Trends in Biochem. Sci. 7:88-92.
21.
Pollard, T.D., Aebi, U., Cooper, J.A., Elzinga, M.,
Fowler, W.E.,
Griffith, L.M., Herman, I.M., Heuser, J., Isenberg, G., Kiehart, D.P.,
Levy,
J., MacLean-Fletcher, S., Maupin, P., Mooseker, M.S., Runge, M., Smith,
P.R.
and Tseng, P. (1982) The mechanism of
actin-filament assembly and cross-linking. In: "Cell and Muscle
Motility", (R.W. Dowben and J.W. Shay, eds.), Plenum Publ. Corp., New York,
2:15-44.
22.
Pollard, T.D. (1982) A falling ball apparatus to
measure filament
cross-linking. In: "Methods in Cell Biology", Vol. 24, Academic
Press, Inc., New York,
pp. 301-311.
23.
Pollard, T.D. (1982) Myosin purification and
characterization.
In: "Methods in Cell Biology", Vol. 24, Academic Press, Inc., New York, pp.
333-371.
24.
Pollard, T.D., Aebi, U., Cooper, J.A., Fowler, W.E.
and Tseng, P.
(1982) Actin structure, polymerization and gelation. Cold Spring
Harbor
Symposia on Quantitative Biology, Vol. 46, Cold
Spring Harbor
Laboratory Press, New York, pp. 513-524.
25.
Pollard, T.D., Griffith,
L.M. and Herman, I.M. (1982) Actin filament-microtubule interactions.
In:
"Proceedings of the ICN-UCLA Symposia. Differentiation and Function of
Hemapoietic Cell Surfaces", (V.T. Marchesi and R.C. Gallo, eds.), Alan
R.
Liss, Inc., New York,
pp. 183-192.
26.
Pollard, T.D. (1982) Assays for myosin. In: "Methods
in
Enzymology" (L.W. Cunningham and D.W. Frederiksen, eds.), Vol. 85,
Academic Press, Inc., New
York,
pp. 123-130.
27.
Cooper, J.A. and Pollard, T.D. (1982) Methods to
measure actin
polymerization. In: "Methods in Enzymology", (L.W. Cunningham and
D.W. Frederiksen, eds.), Vol. 85, Academic Press, Inc., New York, pp.
182-210.
28.
Pollard, T.D. and Cooper, J.A. (1982) Methods to
characterize
actin filament networks. In: "Methods in Enzymology", (L.W.
Cunningham and D.W. Frederiksen, eds.), Vol. 85, Academic Press, Inc., New York, pp.
211-233.
29.
Pollard, T.D. (1982) Purification of nonmuscle
myosins. In:
"Methods in Enzymology, (L.W. Cunningham and D.W. Frederiksen, eds.),
Vol.
85, Academic Press, Inc., New
York,
pp. 331-356.
30.
Pollard, T.D., Aebi, U., Cooper, J.A., Fowler, W.E.,
Kiehart,
D.P., Smith, P.R. and Tseng, P.C.-H. (1982) Actin and myosin function
in Acanthamoeba. Phil. Trans. Royal Soc.
London B 299:237-245.
