Actin filament branching by Arp2/3 complex

Time lapse (50X) total internal reflection microscopy of the polymerization of 2 µM actin (20% labeled with rhodamine on cys-374) with 100 nM Arp2/3 complex, 300 nM Scar VCA. Ref: Amann, K.J. and Pollard, T.D. (2001) Direct real-time observation of actin filament branching mediated by Arp2/3 complex using total internal reflection microscopy. Proc. Nat. Acad. Sci. (U.S.A.) 98:15009-15013.

Last Updated: June 11, 2004