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Use Centrifuges Safely

Below are simple guidelines to follow for the safe operation of a centrifuge in your work area:

  • Each operator should be trained on proper operating procedures.
  • Keep a log book detailing operation records for centrifuges and rotors.
  • DO NOT exceed safe rotor speed.
  • Place a biohazard label on the centrifuge if used for infectious agents.
  • Always use sealed safety cups or sealed rotors with O-rings.
  • Check tubes and bottles for cracks and deformities before each use.
  • Examine O-rings and replace if worn, cracked or missing.
  • Never overfill primary containers (don't exceed 3/4 full).
  • Wipe exterior of tubes or bottles with disinfectant prior to loading.
  • Stop the centrifuge immediately if an unusual condition (noise or vibration) begins.
  • Wait 5 minutes after the run before opening the centrifuge. This will allow aerosols to settle in the event of a breakdown in containment.
  • Decontaminate safety carriers or rotors and centrifuge interior after each use. Wash hands after removing gloves.
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Office of Environmental Health & Safety, 135 College Street, 1st Floor
New Haven, CT 06510-2411 Telephone: 203-785-3550 / Fax: 203-785-7588