Post-doctoral positions
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Postdoctoral positions in the Giraldez Laboratory


In the Giraldez’ lab we combine genetics, embryology, genomics, biochemistry, and computational biology to address a central question in biology: how does a fertilized egg develop into a complex multicellular embryo? in particular we are interested in a long standing problem in developmental biology the maternal to zygotic transition. This universal transition takes place in all animals and consists of two main steps. First, the maternal stages are characterized by a transcriptionally silent zygotic genome, where the first developmental decisions depend on the maternally deposited mRNAs and proteins. Next, activation of the zygotic genome takes place and this triggers the clearance of maternally deposited mRNAs to progress to zygotic stages.

In the Giraldez’ lab we aim to understand: How is the zygotic genome activated? and what are the factors that trigger the decay of maternal mRNAs to undergo zygotic development? To address these questions there are three projects open for postdoctoral positions: based on RNA Biochemistry, Developmental Biology and Computational Biology


These are some of the papers recently published by the laboratory:

  1. 1.Bazzini AA, Lee MT, Giraldez AJ‡. Ribosome Profiling Shows That miR-430 Reduces Translation Before Causing mRNA Decay in Zebrafish. Science 13 April 2012: 233-237.

  2. 2.Staton AA, Knaut H and Giraldez AJ‡. miRNA regulation of SDF1 chemokine signaling provides genetic robustness to germ cell migration. Nature Genetics. 2011 Mar;43(3):204-11. Epub 2011 Jan 23

  3. 3.Cifuentes D, Xue H, Taylor DW, Patnode H, Mishima Y, Cheloufi S, Ma E, Mane S, Hannon GJ, Lawson N, Wolfe S, Giraldez AJ‡. A novel miRNA processing pathway independent of Dicer requires Argonaute2. Science. 2010, Jun 25;328(5986):1694-8. Epub 2010 May 6

  4. 4.Mishima Y, Abreu-Goodger C, Staton AA, Stahlhut C, Shou C, Cheng C, Gerstein M, Enright AJ and Giraldez AJ. Zebrafish miR-1 and miR-133 shape muscle gene expression and regulate sarcomeric actin organization. Genes & Development. 2009 Mar 1;23(5):619-32. Epub 2009 Feb 24. PMID: 19240126

  5. 5.Choi WY, Giraldez AJ, Schier AF. Target Protectors Reveal Dampening and Balancing of Nodal Agonist and Antagonist by miR-430. Science. 2007. Oct 12;318(5848):271-4. Corresponding authors.

  6. 6.Giraldez AJ‡, Mishima Y, Rihel J, Grocock R, van Dongen S, Inoue, K, Enright A, and Schier AF‡. Zebrafish miR-430 promotes deadenylation and clearance of maternal mRNAs. Science. 2006 Apr 7;312(5770):75-9.


Description of the positions:


RNA Biochemist

Research Area: RNA biochemistry, RNA protein interactions, PAR-clip, Mass spectrometry

Project title: Identification of the protein-RNA interactions that mediate post-transcriptional regulation in development

Description of project:  A postdoctoral position is available to identify and characterize RNA-protein interactions that regulate mRNA decay and translational regulation during vertebrate development.

Applicants require a strong background RNA and protein biochemistry

Application:

Please e-mail CV, summary and relevance of your current research (500 word max), why you are interested in the giraldez lab (200 word max) and names of three references to: giraldezlab@gmail.com Include “RNA Biochemistry postdoctoral applicant” in the subject of the email


Developmental Biologist

Research Area: The maternal to zygotic transition


Project title: regulatory networks that control maternal mRNA decay and zygotic genome activation


Description of project: A postdoctoral position is available to understand the regulatory interactions that control maternal mRNA decay and zygotic genome activation

Applicants require a strong background in genetics and developmental biology using a model system.

Application:

Please e-mail CV, summary and relevance of your current research (500 word max), why you are interested in the giraldez lab (200 word max) and names of three references to: giraldezlab@gmail.com Include “Developmental Biology postdoctoral applicant” in the subject of the email

Computational Biologist

Research Area:  non-coding RNAs, RNA-seq, Ribosome profiling, Par-Clip, Gene expression analysis


Description of project: Several postdoctoral position in computational biology are available to study:

  1. -The regulatory motifs and the RNA binding proteins that control translation, transcription and mRNA decay during development.


  1. -Small RNas and other non-coding small RNAs by combining high throughput sequencing and genetics in vertebrate model systems.


The giraldez laboratory provides an integrated environment for computational biologists and experimental biologists to develop these projects.

Applicants require a strong background in Bioinformatics, sequence analysis, statistics and perl programming.  There are other two computational biologists in the Giraldez laboratory.


Application:

Please e-mail CV, summary and relevance of your current research (500 word max), why you are interested in the giraldez lab (200 word max) and names of three references to: giraldezlab@gmail.com

Include “Computational Biology 2012” in the subject of the email


These are some of the papers recently published by the laboratory:

  1. 1.Bazzini AA, Lee MT, Giraldez AJ‡. Ribosome Profiling Shows That miR-430 Reduces Translation Before Causing mRNA Decay in Zebrafish. Science 13 April 2012: 233-237.

  2. 2.Staton AA, Knaut H and Giraldez AJ‡. miRNA regulation of SDF1 chemokine signaling provides genetic robustness to germ cell migration. Nature Genetics. 2011 Mar;43(3):204-11. Epub 2011 Jan 23

  3. 3.Cifuentes D, Xue H, Taylor DW, Patnode H, Mishima Y, Cheloufi S, Ma E, Mane S, Hannon GJ, Lawson N, Wolfe S, Giraldez AJ‡. A novel miRNA processing pathway independent of Dicer requires Argonaute2. Science. 2010, Jun 25;328(5986):1694-8. Epub 2010 May 6

  4. 4.Mishima Y, Abreu-Goodger C, Staton AA, Stahlhut C, Shou C, Cheng C, Gerstein M, Enright AJ and Giraldez AJ. Zebrafish miR-1 and miR-133 shape muscle gene expression and regulate sarcomeric actin organization. Genes & Development. 2009 Mar 1;23(5):619-32. Epub 2009 Feb 24. PMID: 19240126

  5. 5.Choi WY, Giraldez AJ‡, Schier AF. Target Protectors Reveal Dampening and Balancing of Nodal Agonist and Antagonist by miR-430. Science. 2007. Oct 12;318(5848):271-4. Corresponding authors.

  6. 6.Giraldez AJ‡, Mishima Y, Rihel J, Grocock R, van Dongen S, Inoue, K, Enright A, and Schier AF‡. Zebrafish miR-430 promotes deadenylation and clearance of maternal mRNAs. Science. 2006 Apr 7;312(5770):75-9.

Thanks for checking out our postings for positions:

We are a lab of scientists who are passionate about what we do, with interests and expertise spanning Development, Biochemistry, Genomics, and Computational biology. Our projects are focused on collaborative, integrative science to study the earliest steps of vertebrate development. We are looking for scientists who share our passion for science and would like to work in a friendly, multidisciplinary and multicultural environment.