Clean Up of a Biosafety
Level 2 Spill

• Allow aerosols to disperse for at least 30 minutes before reentering the laboratory.
• Assemble clean-up materials (disinfectant, paper towels, biohazard bags, and forceps).
• Cover the area with disinfectant-soaked towels, and then carefully pour disinfectant around the spill. Avoid enlarging the contaminated area. Use more concentrated disinfectant as it is diluted by the spill. Allow at least a 20 minute contact time.
• Handle any sharps objects with forceps and discard in a sharps container. Wipe surrounding areas (where the spill may have splashed) with disinfectant. Soak up the disinfectant and spill, and place the materials into a biohazard bag.
• Spray the area with 10% household bleach solution and allow to air-dry (or wipe down with disinfectant-soaked towels after a 10 minute contact time). Place all contaminated paper towels and any contaminated protective clothing into a biohazard bag and autoclave.
• Wash hands and exposed skin areas with antiseptic soap and water.